Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Correspondence
  • Published:

Reply to 'Mechanism for microtsunami-induced intercellular mechanosignalling'

Nature Photonics volume 9, pages 624–625 (2015)Cite this article

Subjects

Luo et al. reply

We thank He et al.1 for their Correspondence regarding our recent paper2. They offer an alternate hypothesis for our observed microtsunami (μtsunami)-initiated Ca2+ signalling (μT-ICS) that differs from our claim of mechanical activation of stretch-sensitive G protein-coupled receptors (SS-GPCRs) by shear flow. Instead, they assert that μtsunami exposure initiates the release of Ca2+ signalling molecules, including ATP, from the cell immediately below the site of laser radiation. These molecules then diffuse to neighbouring cells and activate purinergic receptors, including those coupled to GPCRs, resulting in a propagating intercellular Ca2+ wave. They cite previous studies3,4,5 that use femtosecond-laser microbeam irradiation to initiate Ca2+ signalling in primary rat hippocampal cells and astrocytes as well as immortalized human kidney (HEK293T) and cervical cancer (HeLa) cells. These Ca2+ waves have higher velocities (10–40 μm s−1) and smaller spatial extent (∼200 μm radius) as compared with μT-ICS waves (4.5 μm s−1 and ∼400 μm radius, respectively) reported in our paper. Importantly, our studies employed human vascular endothelial cells (HUVECs) whose physiological function requires mechanosensitivity to hemodynamic shear stress.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Mechanical dose-response of μT-ICS.

References

  1. He, H., Nakagawa, K., Wang Y., Hosokawa, Y. and Goda, K. Nature Photon. 9, 623 (2015).

    Article  ADS  Google Scholar 

  2. Compton, J. L., Luo, J. C., Ma, H., Botvinick, E. & Venugopalan V. Nature Photon. 8, 710–715 (2014).

    Article  ADS  Google Scholar 

  3. He, H. et al. Appl. Phys. Lett. 100, 173704 (2012).

    Article  ADS  Google Scholar 

  4. Liu, X., Lv, X., Zeng, S., Zhou, W. & Luo, Q. Appl. Phys. Lett. 94, 061113 (2009).

    Article  ADS  Google Scholar 

  5. Zhao, Y. et al. J. Biomed. Opt. 15, 035001 (2010).

    Article  ADS  Google Scholar 

  6. Jacobson, K. A. P2X and P2Y Receptors (Tocris Bioscience, 2010); http://www.tocris.com/pdfs/pdf_downloads/Purinergic_Receptors_Review.pdf

    Google Scholar 

  7. Kaczmarek, E. in Extracellular ATP and Adenosine as Regulators of Endothelial Cell Function (eds Gerasimovskaya, E. & Kaczmarek, E.) Ch. 2 (Springer, 2010).

    Google Scholar 

  8. Erlinge, D. & Burnstock, G. Purinergic Signal. 4, 1–20 (2008).

    Article  Google Scholar 

  9. Bishara, N. B., Murphy, T. V. & Hill, M. A. Br. J. Pharmacol. 135, 119–128 (2002).

    Article  Google Scholar 

  10. Tirlapur, U. K. & König, K Nature 418, 290–291 (2002).

    Article  ADS  Google Scholar 

  11. Vogel, A., Noack, J., Hüttman, G. & Paltauf, G. Appl. Phys. B 81, 1015–1047 (2005).

    Article  ADS  Google Scholar 

  12. Quinto-Su, P. A. & Venugopalan, V. Methods Cell Biol. 82, 111–151 (2007).

    Article  Google Scholar 

  13. Compton, J. L., Hellman, A. N. & Venugopalan, V. Biophys. J. 105, 2221–2231 (2013).

    Article  ADS  Google Scholar 

  14. Hellman, A. N., Rau, K. R., Yoon, H. H. & Venugopalan, V. J. Biophoton. 1, 24–35 (2008).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biomedical Engineering, University of California, Irvine, Irvine, 92697, California, USA

    Justin C. Luo, Elliot L. Botvinick & Vasan Venugopalan

  2. Laser Microbeam and Medical Program, Beckman Laser Institute, University of California, Irvine, Irvine, 92697, California, USA

    Justin C. Luo, Elliot L. Botvinick & Vasan Venugopalan

  3. Edwards Lifesciences Center for Advanced Cardiovascular Technology, University of California, Irvine, Irvine, 92697, California, USA

    Elliot L. Botvinick

  4. Department of Chemical Engineering and Materials Science, University of California, Irvine, Irvine, 92697, California, USA

    Vasan Venugopalan

Authors
  1. Justin C. Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Elliot L. Botvinick
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Vasan Venugopalan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Elliot L. Botvinick or Vasan Venugopalan.

Supplementary information

Supplementary information

Supplementary information (PDF 583 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Luo, J., Botvinick, E. & Venugopalan, V. Reply to 'Mechanism for microtsunami-induced intercellular mechanosignalling'. Nature Photon 9, 624–625 (2015). https://doi.org/10.1038/nphoton.2015.165

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nphoton.2015.165

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing