An imaging platform based on broadband coherent anti-Stokes Raman scattering has been developed that provides an advantageous combination of speed, sensitivity and spectral breadth. The system utilizes a configuration of laser sources that probes the entire biologically relevant Raman window (500–3,500 cm–1) with high resolution (<10 cm–1). It strongly and efficiently stimulates Raman transitions within the typically weak ‘fingerprint’ region using intrapulse three-colour excitation, and utilizes the non-resonant background to heterodyne-amplify weak Raman signals. We demonstrate high-speed chemical imaging in two- and three-dimensional views of healthy murine liver and pancreas tissues as well as interfaces between xenograft brain tumours and the surrounding healthy brain matter.
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The authors thank Q. Wu, J. Hale and M. Sinyuk for preparing the pathological tissue specimens and S. Miller for preparation of neat chemical specimens. C.H.C., J.M.H. and C.M.H. also thank the National Research Council for support through the Research Associate Program (RAP). This work was supported in part by NIH/NIBIB grant 2P41EB001046-11.
The authors declare no competing financial interests.
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Camp Jr, C., Lee, Y., Heddleston, J. et al. High-speed coherent Raman fingerprint imaging of biological tissues. Nature Photon 8, 627–634 (2014). https://doi.org/10.1038/nphoton.2014.145
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