Nonlinear microscopy techniques developed over the past two decades have provided dramatic new capabilities for biological imaging. The initial demonstrations of nonlinear microscopies coincided with the development of solid-state femtosecond lasers, which continue to be the dominant light source for applications of nonlinear microscopy. Fibre lasers offer attractive features for biological and biomedical imaging, and recent advances are promising for the development of high-performance sources with the potential for realizing integrated instruments that are robust and inexpensive. This Review discusses recent advances, and identifies challenges and opportunities for fibre lasers in nonlinear bioimaging.
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Portions of this work were supported by the National Institutes of Health (EB002019, R01CA133148, R01EB014873 and R21RR032392) and the National Science Foundation (ECCS-0901323, BIS-0967949).
F.Â W. Wise is a named inventor on US patent US 8,416,817 B2 (publication date 04.09.2008, filing date 18.09.2007) and Chinese patent number 200780042670.8, which are related to the dissipative-soliton laser described in this Review Article. European patent application number 7873804.4 has been filed on the same subject. Wise has also submitted a patent application relating to picosecond-pulse sources for coherent anti-Stokes Raman microscopy (international patent PCT/US/2012/058817 (publication date 11.04.2013, filing date 04.10.2011).
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Xu, C., Wise, F. Recent advances in fibre lasers for nonlinear microscopy. Nature Photon 7, 875–882 (2013). https://doi.org/10.1038/nphoton.2013.284
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