Multiphoton microscopy has enabled unprecedented dynamic exploration in living organisms. A significant challenge in biological research is the dynamic imaging of features deep within living organisms, which permits the real-time analysis of cellular structure and function. To make progress in our understanding of biological machinery, optical microscopes must be capable of rapid, targeted access deep within samples at high resolution. In this Review, we discuss the basic architecture of a multiphoton microscope capable of such analysis and summarize the state-of-the-art technologies for the quantitative imaging of biological phenomena.
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The authors recognize the support of the National Institute of Biomedical Imaging and Bioengineering under the Bioengineering Research Partnership EB-003832. In addition, the authors thank E. Toberer for his assistance in preparing portions of this manuscript.
The authors declare no competing financial interests.
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Hoover, E., Squier, J. Advances in multiphoton microscopy technology. Nature Photon 7, 93–101 (2013). https://doi.org/10.1038/nphoton.2012.361
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