Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Commentary
  • Published:

Nano-imaging with STORM

A Correction to this article was published on 01 August 2009

This article has been updated

Multicolour, three-dimensional stochastic optical reconstruction microscopy now makes it possible to image cellular structures with near molecular-scale resolution.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Figure 1: The principle of STORM.
Figure 2: STORM imaging of cells with photo-switchable dyes and fluorescent proteins.
Figure 3: Multicolour STORM.
Figure 4: Three-dimensional STORM.

Change history

  • 02 July 2009

    In the version of this Commentary originally published, the author name and volume number of reference 28 were incorrect and should have been: Pavani, S. R. P. et al. Proc. Natl Acad. Sci. USA 106, 2995–2999 (2009).This reference has been corrected in the HTML and PDF versions of the Commentary.

References

  1. Hell, S. W., Schmidt, R. & Egner, A. Nature Photon. 3, 381–387 (2009).

    Article  ADS  Google Scholar 

  2. Heintzmann, R. & Gustafsson, M. G. L. Nature Photon. 3, 362–364 (2009).

    Article  ADS  Google Scholar 

  3. Rust, M. J, Bates, M. & Zhuang, X. Nature Meth. 3, 793–795 (2006).

    Article  Google Scholar 

  4. Betzig, E. et al. Science 313, 1642–1645 (2006).

    Article  ADS  Google Scholar 

  5. Hess, S. T., Girirajan, T. P. K. & Mason, M. D. Biophys. J. 91, 4258–4272 (2006).

    Article  ADS  Google Scholar 

  6. Moerner, W. E. & Orrit, M. Science 283, 1670–1676 (1999).

    Article  ADS  Google Scholar 

  7. Gelles, J., Schnapp, B. J. & Sheetz, M. P. Nature 331, 450–453 (1988).

    Article  ADS  Google Scholar 

  8. Thompson, R. E., Larson, D. R. & Webb, W. W. Biophys. J. 82, 2775–2783 (2002).

    Article  Google Scholar 

  9. Yildiz, A. et al. Science 300, 2061–2065 (2003).

    Article  ADS  Google Scholar 

  10. Bates, M., Blosser, T. R. & Zhuang, X. Phys. Rev. Lett. 94, 108101 (2005).

    Article  ADS  Google Scholar 

  11. Heilemann, M., Margeat, M., Kasper, R., Sauer, M. & Tinnefeld, P. J. Am. Chem. Soc. 127, 3801–3806 (2005).

    Article  Google Scholar 

  12. Bates, M., Huang, B., Dempsey, G. T. & Zhuang, X. Science 317, 1749–1753 (2007).

    Article  ADS  Google Scholar 

  13. Heilemann, M. et al. Angew. Chem. Int. Ed. 47, 6172–6176 (2008).

    Article  Google Scholar 

  14. Folling, J. et al. Angew. Chem. Int. Ed. 46, 6266–6270 (2007).

    Article  Google Scholar 

  15. Fölling, J. et al. Nature Meth. 5, 943–945 (2008).

    Article  Google Scholar 

  16. Steinhauer, C., Forthmann, C., Vogelsang, J. & Tinnefeld, P. J. Am. Chem. Soc. 130, 16840–16841 (2008).

    Article  Google Scholar 

  17. McKinney, S. A., Murphy, C. S., Hazelwood, K. L., Davidson, M. W. & Looger, L. L. Nature Meth. 6, 131–133 (2008).

    Article  Google Scholar 

  18. Wiedenmann, J. et al. Proc. Natl Acad. Sci. USA 101, 15905–15910 (2004).

    Article  ADS  Google Scholar 

  19. Egner, A. et al. Biophys. J. 93, 3285–3290 (2007).

    Article  ADS  Google Scholar 

  20. Shroff, H. et al. Proc. Natl Acad. Sci. USA 104, 20308–20313 (2007).

    Article  ADS  Google Scholar 

  21. Andresen, M. et al. Nature Biotechnol. 26, 1035–1040 (2008).

    Article  Google Scholar 

  22. Biteen, J. S. et al. Nature Meth. 5, 947–949 (2008).

    Article  Google Scholar 

  23. Subach, F. V. et al. Nature Meth. 6, 153–159 (2009).

    Article  ADS  Google Scholar 

  24. Bock, H. et al. Appl. Phys. B 88, 161–165 (2007).

    Article  ADS  Google Scholar 

  25. Huang, B., Jones, S. A., Brandenburg, B. & Zhuang, X. Nature Meth. 5, 1047–1052 (2008).

    Article  Google Scholar 

  26. Huang, B., Wang, W., Bates, M. & Zhuang, X. Science 319, 810–813 (2008).

    Article  ADS  Google Scholar 

  27. Juette, M. F. et al. Nature Meth. 5, 527–529 (2008).

    Article  Google Scholar 

  28. Pavani, S. R. P. et al. Proc. Natl Acad. Sci. USA 106, 2995–2999 (2009).

    Article  ADS  Google Scholar 

  29. Shtengel, G. et al. Proc. Natl Acad. Sci. USA 106, 3125–3130 (2009).

    Article  ADS  Google Scholar 

  30. Huang, B., Bates, M. & Zhuang, X. Annu. Rev. Biochem. 78, 993–1016 (2009).

    Article  Google Scholar 

  31. Fernandez-Suarez, M. & Ting, A. Y. Nature Rev. Mol. Cell. Biol. 9, 929–943 (2008).

    Article  Google Scholar 

  32. Shroff, H., Galbraith, C. G., Galbraith, J. A. & Betzig, E. Nature Meth. 5, 417–423 (2008).

    Article  Google Scholar 

Download references

Acknowledgements

This work is supported in part by the National Institutes of Health and Howard Hughes Medical Institute. I thank B. Huang and G. Dempsey for taking the images shown in Fig. 2.

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhuang, X. Nano-imaging with STORM. Nature Photon 3, 365–367 (2009). https://doi.org/10.1038/nphoton.2009.101

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/nphoton.2009.101

This article is cited by

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing