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Enhancing nanopore sensing with DNA nanotechnology

Nature Nanotechnology volume 11, pages 106–108 (2016)Cite this article

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Nanopores are on the brink of fundamentally changing DNA sequencing. At the same time, DNA origami provides unprecedented freedom in molecular design. Here, I suggest why a combination of solid-state nanopores and DNA nanotechnology will lead to exciting new experiments.

There are a number of key advantages of resistive-pulse sensing. It is, for example, inherently label-free as the analyte is detected by the exclusion of ions from the sensing volume defined by the nanopore. Its spatial resolution is, in principle, only determined by the dimensions of the nanopore. Its detection principle suggests it could be used as a single sensor for any molecular object that leads to a detectable current signature. Miniaturization is straightforward, as nanopore sensing avoids any optical means, and is easy to integrate into highly portable lab-on-a-chip devices.

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Figure 1: Challenges of label-free nanopore sensing.
Figure 2: Combining DNA nanotechnology and nanopores.

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  1. Ulrich F. Keyser is at the Cavendish Laboratory, University of Cambridge, JJ Thomson Avenue, Cambridge CB3 0HE, UK,

    Ulrich F. Keyser

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  1. Ulrich F. Keyser
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Correspondence to Ulrich F. Keyser.

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The author's research is partly funded by Oxford Nanopore Technologies.

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Keyser, U. Enhancing nanopore sensing with DNA nanotechnology. Nature Nanotech 11, 106–108 (2016). https://doi.org/10.1038/nnano.2016.2

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