Tensegrity, or tensional integrity, is a property of a structure indicating a reliance on a balance between components that are either in pure compression or pure tension for stability1,2. Tensegrity structures exhibit extremely high strength-to-weight ratios and great resilience, and are therefore widely used in engineering, robotics and architecture3,4. Here, we report nanoscale, prestressed, three-dimensional tensegrity structures in which rigid bundles of DNA double helices resist compressive forces exerted by segments of single-stranded DNA that act as tension-bearing cables. Our DNA tensegrity structures can self-assemble against forces up to 14 pN, which is twice the stall force of powerful molecular motors such as kinesin or myosin5,6. The forces generated by this molecular prestressing mechanism can be used to bend the DNA bundles or to actuate the entire structure through enzymatic cleavage at specific sites. In addition to being building blocks for nanostructures, tensile structural elements made of single-stranded DNA could be used to study molecular forces, cellular mechanotransduction and other fundamental biological processes.
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Snelson, K. Snelson on the tensegrity invention. Int. J. Space Struct. 11, 43–48 (1996).
Fuller, B. Synergetics—Explorations in the Geometry of Thinking Vols I and II (Macmillan Publishing, 1975, 1979).
Skelton, R. E., Adhikari, R., Pinaud, J. P. & Chan, W. An introduction to the mechanics of tensegrity structures. Proceedings of the 40th IEEE Conference on Decision and Control 5, 4254–4259 (2001).
Sultan, C. Corless, M. & Skelton, R. E. The prestressabilty problem of tensegrity structures: some analytical solutions. Int. J. Solids Struct. 38, 5223–5252 (2001).
Visscher, K., Schnitzer, M. J. & Block, S. M. Single kinesin molecules studied with a molecular force clamp. Nature 400, 184–189 (1999).
Clemen, A. E. et al. Force-dependent stepping kinetics of myosin-V. Biophys. J. 88, 4402–4410 (2005).
Ingber, D. E. The architecture of life. Sci. Am. 278, 48–57 (1998).
Ingber, D. E. Cellular mechanotransduction: putting all the pieces together again. FASEB J. 20, 811–827 (2006).
Shih, W. M., Quispe J. D. & Joyce, G. F. A 1.7-kilobase single-stranded DNA that folds into a nanoscale octahedron. Nature 427, 618–621 (2004).
Liu, D., Wang, M. S., Deng, Z. X., Walulu, R. & Mao, C. D. Tensegrity: construction of rigid DNA triangles with flexible four-arm DNA junctions. J. Am. Chem. Soc. 126, 2324–2325 (2004).
Zhang, C. et al. Conformational flexibility facilitates self-assembly of complex DNA nanostructures. Proc. Natl Acad. Sci. 31, 10665–10669 (2008).
He, Y. et al. Hierarchical self-assembly of DNA into symmetric supramolecular polyhedra. Nature 452, 198–201 (2008).
Zheng, J. et al. From molecular to macroscopic via the rational design of a self-assembled 3D DNA crystal. Nature 461, 74–77 (2009).
Rothemund, P. W. K. Folding DNA to create nanoscale shapes and patterns. Nature 440, 287–302 (2006).
Douglas, S. M. et al. Self-assembly of DNA into nanoscale three-dimensional shapes. Nature 459, 414–418 (2009).
Smith, S. B., Cui, Y. J. & Bustamante, C. Overstretching B-DNA: the elastic response of individual double-stranded and single-stranded DNA molecules. Science 271, 795–799 (1996).
Douglas, S. M. et al. Rapid prototyping of three-dimensional DNA-origami shapes with caDNAno. Nucleic Acids Res. 37, 5001–5006 (2009).
Marko, J. F. & Siggia, E. D. Stretching DNA. Macromolecules 28, 8759–8770 (1995).
Douglas, S. M., Chou, J. J. & Shih, W. M. DNA-nanotube-induced alignment of membrane proteins for NMR structure determination. Proc. Natl Acad. Sci. USA 104, 6644–6648 (2007).
Howard, J. Mechanics of Motor Proteins and the Cytoskeleton (Sinauer Associates, 2001).
Goodman, R. P. et al. Rapid chiral assembly of rigid DNA building blocks for molecular nanofabrication. Science 310, 1661–1665 (2005).
Saleh, O. A., McIntosh, D. B., Pincus, P. & Ribeck, N. Nonlinear low-force elasticity of single-stranded DNA molecules. Phys. Rev. Lett. 102, 068301 (2009).
Högberg, B., Liedl, T. & Shih, W. M. Folding DNA origami from a double-stranded source of scaffold. J. Am. Chem. Soc. 131, 91544–94155 (2009).
Ingber, D. E. The origin of cellular life. Bioessays 22, 1160–1167 (2000).
Shroff, H. et al. Biocompatible force sensor with optical readout and dimensions of 6 nm3. Nano Lett. 5, 1509–1514 (2005).
The authors thank O. Hallatschek, R. Neher, H. Dietz and S. Douglas for helpful discussions and advice. This work was funded by the Wyss Institute for Biologically Inspired Engineering, and Deutscher Akademischer Austauschdienst (DAAD; T.L.), Swedish Science Council (Vetenskapsrådet) Fellowship (B.H.) and Claudia Adams Barr Program Investigator and NIH New Innovator (1DP2OD004641-01; W.M.S.) grants.
The authors declare no competing financial interests.
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Advanced Materials (2019)
Nature Chemical Biology (2019)
ACS Nano (2019)