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TARP γ-8 controls hippocampal AMPA receptor number, distribution and synaptic plasticity

Nature Neuroscience volume 8, pages 15251533 (2005) | Download Citation

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Abstract

Synaptic plasticity involves activity-dependent trafficking of AMPA-type glutamate receptors. Numerous cytoplasmic scaffolding proteins are postulated to control AMPA receptor trafficking, but the detailed mechanisms remain unclear. Here, we show that the transmembrane AMPA receptor regulatory protein (TARP) γ-8, which is preferentially expressed in the mouse hippocampus, is important for AMPA receptor protein levels and extrasynaptic surface expression. By controlling the number of AMPA receptors, γ-8 is also important in long-term potentiation, but not long-term depression. This study establishes γ-8 as a critical protein for basal AMPA receptor expression and localization at extrasynaptic sites in the hippocampus and raises the possibility that TARP-dependent control of AMPA receptors during synapse development and plasticity may be widespread.

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Acknowledgements

We thank Y.-X. Wang for help in the immunogold studies, P. Seeburg for the GluRA−/− (GluR1−/−) mice and A. Tzingounis for discussion and reading the paper. This research was supported by grants (to D.S.B. and R.A.N) from the National Institutes of Health (NIH), the Howard Hughes Medical Institute Research Resources Program (to D.S.B.) and the Human Frontier Research Program (to D.S.B.). R.S.P. is supported by the Intramural Research Program of the NIH/NIDCD (National Institute on Deafness and Other Communication Disorders). R.A.N. is a member of the Keck Center for Integrative Neuroscience and the Silvo Conte Center for Neuroscience Research. D.S.B. is an established investigator for the American Heart Association. N.R. is supported by a fellowship from the International Human Frontier Science Program Organization. K.B. and S.T. are supported by postdoctoral fellowships from NIH.

Author information

Author notes

    • Nathalie Rouach
    •  & Keith Byrd

    These authors contributed equally to this work.

    • Nathalie Rouach

    Present address: Laboratory of Neuropharmacology, Collège de France, 11 place Marcelin Berthelot, 75005 Paris, France.

Affiliations

  1. Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California 94143, USA.

    • Nathalie Rouach
    • , Guillermo M Elias
    • , Hillel Adesnik
    • , Siavash Karimzadegan
    • , Colin Kealey
    •  & Roger A Nicoll
  2. Department of Physiology, University of California, San Francisco, California 94143, USA.

    • Keith Byrd
    • , Susumu Tomita
    • , David S Bredt
    •  & Roger A Nicoll
  3. Laboratory of Neurochemistry, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, Maryland 20892, USA.

    • Ronald S Petralia

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The authors declare no competing financial interests.

Corresponding author

Correspondence to Roger A Nicoll.

Supplementary information

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  1. 1.

    Supplementary Fig. 1

    Diagram depicting amount of GluR1 in the synaptic, extrasynaptic and cytoplasmic regions of hippocampal spines from γ-8−/− and γ-8+/+ mice.

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    Supplementary Methods

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DOI

https://doi.org/10.1038/nn1551

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