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Retinal network adaptation to bright light requires tyrosinase

Nature Neuroscience volume 7, pages 13291336 (2004) | Download Citation

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Abstract

The visual system adjusts its sensitivity to a wide range of light intensities. We report here that mutation of the zebrafish sdy gene, which encodes tyrosinase, slows down the onset of adaptation to bright light. When fish larvae were challenged with periods of darkness during the day, the sdy mutants required nearly an hour to recover optokinetic behavior after return to bright light, whereas wild types recovered within minutes. This behavioral deficit was phenocopied in fully pigmented fish by inhibiting tyrosinase and thus does not depend on the absence of melanin pigment in sdy. Electroretinograms showed that the dark-adapted retinal network recovers sensitivity to a pulse of light more slowly in sdy mutants than in wild types. This failure is localized in the retinal neural network, postsynaptic to photoreceptors. We propose that retinal pigment epithelium (which normally expresses tyrosinase) secretes a modulatory factor, possibly L-DOPA, which regulates light adaptation in the retinal circuitry.

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Acknowledgements

We thank J. Kay, A. Wehman, S. Taha, and M. Orger for comments on the manuscript and all members of our laboratory for discussions. We also thank A. Churchland and L. Gitlin for assistance in the initial identification of zebrafish tyrosinase. This study was supported by a NARSAD Young Investigator award and the UCSF Neuroscience training grant (P.P.-M.), a B.I.F. fellowship (T.R.), the Packard Foundation, the Sloan Foundation and the NIH (H.B.).

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Author notes

    • Patrick S Page-McCaw
    •  & Tobias Roeser

    Present addresses: Rensselaer Polytechnic Institute, Troy, New York 12180, USA (P.P.-M.) and Isenbruck and Partners, Technologiepark Heidelberg, Im Neuenheimer Feld 582, 69120 Heidelberg, Germany (T.R.).

    • Patrick S Page-McCaw
    •  & S Clare Chung

    These authors contributed equally to this work.

Affiliations

  1. University of California, San Francisco, Department of Physiology, Program in Neuroscience, 513 Parnassus Ave., San Francisco, California 94143-0444, USA.

    • Patrick S Page-McCaw
    • , S Clare Chung
    • , Akira Muto
    • , Tobias Roeser
    • , Wendy Staub
    • , Karin C Finger-Baier
    • , Juan I Korenbrot
    •  & Herwig Baier

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The authors declare no competing financial interests.

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Correspondence to Herwig Baier.

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DOI

https://doi.org/10.1038/nn1344

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