Towing of sensory axons by their migrating target cells in vivo

Abstract

Many pathfinding axons must locate target fields that are themselves positioned by active migration. A hypothetical method for ensuring that these migrations are coordinated is towing, whereby the extension of axons is entirely dependent on the migration of their target cells. Here we combine genetics and time-lapse imaging in the zebrafish to show that towing by migrating cells is a bona fide mechanism for guiding pathfinding axons in vivo.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

Figure 1: In vivo imaging of lateral line migration in wild-type and ody mutants.
Figure 2: The lateral line nerve is towed by the migrating primordium.
Figure 3: Migrating LLP provides cues that are sufficient to guide the LL nerve.

References

  1. 1

    Tessier-Lavigne, M. & Goodman, C.S. Science 274, 1123–1133 (1996).

  2. 2

    Hatten, M.E. Annu. Rev. Neurosci. 22, 511–539 (1999).

  3. 3

    Weiss, P. Third Growth Symposium 5, 163–203 (1941).

  4. 4

    Metcalfe, W.K. J. Comp. Neurol. 238, 218–224 (1985).

  5. 5

    Pauls, S., Geldmacher-Voss, B. & Campos-Ortega, J.A. Dev. Genes Evol. 211, 603–610 (2001).

  6. 6

    Knaut, H., Werz, C., Geisler, R. & Nusslein-Volhard, C. Nature 421, 279–282 (2003).

  7. 7

    Doitsidou, M. et al. Cell 111, 647–659 (2002).

  8. 8

    David, N.B. et al. Proc. Natl. Acad. Sci. USA 99, 16297–16302 (2002).

  9. 9

    Zhu, Y. et al. Nat. Neurosci. 5, 719–720 (2002).

  10. 10

    Xiang, Y. et al. Nat. Neurosci. 5, 843–848 (2002).

  11. 11

    Chalasani, S.H., Sabelko, K.A., Sunshine, M.J., Littman, D.R. & Raper, J.A. J. Neurosci. 23, 1360–1371 (2003).

  12. 12

    Andermann, P., Ungos, J. & Raible, D.W. Dev. Biol. 251, 45–58 (2002).

  13. 13

    Shoji, W., Yee, C.S. & Kuwada, J.Y. Development 125, 1275–1283 (1998).

  14. 14

    Gilmour, D.T., Maischein, H.M. & Nusslein-Volhard, C. Neuron 34, 577–588 (2002).

  15. 15

    Becker, T., Becker, C.G., Schachner, M. & Bernhardt, R.R. Mech. Dev. 109, 37–49 (2001).

Download references

Acknowledgements

We are grateful to J. Campos-Ortega for providing the H2A-F/Z:GFP transgenic line and to F. Peri for discussion and critical reading of the manuscript. This work was supported by the Max-Planck-Gesellschaft.

Author information

Correspondence to Darren Gilmour.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Supplementary Fig. 1

(a) In situ hybridisation showing CXCR4b expression in wild type lateral line primordium at 36 hpf. The G-protein coupled receptor is rapidly down regulated in newly deposited pro-neuromasts (arrows). (b) Endogenous alkaline phosphatase staining of neuromasts in 5 day old larvae. Ody mutants (lower) show strongly reduced number of neuromasts in the posterior lateral line when compared to wild type siblings (upper). (JPG 55 kb)

Supplementary Methods (PDF 8 kb)

Rights and permissions

Reprints and Permissions

About this article

Further reading