An evolutionarily conserved dileucine motif in Shal K+ channels mediates dendritic targeting

  • A Corrigendum to this article was published on 01 August 2003

Abstract

The molecular mechanisms underlying polarized sorting of proteins in neurons are poorly understood. Here we report the identification of a 16 amino-acid, dileucine-containing motif that mediates dendritic targeting in a variety of neuronal cell types in slices of rat brain. This motif is present in the carboxy (C) termini of Shal-family K+ channels and is highly conserved from C. elegans to humans. It is necessary for dendritic targeting of potassium channel Kv4.2 and is sufficient to target the axonally localized channels Kv1.3 and Kv1.4 to the dendrites. It can also mediate dendritic targeting of a non-channel protein, CD8.

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Figure 1: Characterization of tagged K+ channel constructs.
Figure 2: Subcellular localization of K+ channel constructs in cortical pyramidal cells in slices.
Figure 3: Alignment of Shal K+ channels reveals a conserved C-terminal dileucine-containing motif that is necessary for dendritic localization.
Figure 4: The dileucine-containing motif is sufficient to mediate dendritic localization in cortical pyramidal neurons.
Figure 5: The dileucine-containing motif mediates dendritic localization of CD8 in cortical pyramidal neurons.
Figure 6: The dileucine-containing motif functions in multiple cell types.
Figure 7: Mutation of the dileucine-containing motif does not affect the rate of endocytosis.

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Acknowledgements

We thank D. Liu for technical assistance, G. Banker for the CD8 expression construct, K. Moriyoshi for pCA–GAP–GFP and C-P. Ko and S. Bottjer for helpful suggestions on the manuscript. This work was supported by National Institutes of Health grants NS41963 (D.B.A.) and DC04213 (E.R.L.) and a grant from the Whitehall foundation (D.B.A.).

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Correspondence to Don B. Arnold.

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