The neocortex generates periods of recurrent activity, such as the slow (0.1–0.5 Hz) oscillation during slow-wave sleep. Here we demonstrate that slices of ferret neocortex maintained in vitro generate this slow (< 1 Hz) rhythm when placed in a bathing medium that mimics the extracellular ionic composition in situ. This slow oscillation seems to be initiated in layer 5 as an excitatory interaction between pyramidal neurons and propagates through the neocortex. Our results demonstrate that the cerebral cortex generates an ‘up’ or depolarized state through recurrent excitation that is regulated by inhibitory networks, thereby allowing local cortical circuits to enter into temporarily activated and self-maintained excitatory states. The spontaneous generation and failure of this self-excited state may account for the generation of a subset of cortical rhythms during sleep.
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We thank L. G. Nowak for participation in critical portions of these experiments. We thank L. Nowak, A. Luthi, J. Brumberg, H. Blumenfeld and R. Gallego for comments on the manuscript. This work was supported by the NIH and the McKnight Foundation. For movies and additional information see http://www.mccormicklab.org.
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Sanchez-Vives, M., McCormick, D. Cellular and network mechanisms of rhythmic recurrent activity in neocortex. Nat Neurosci 3, 1027–1034 (2000). https://doi.org/10.1038/79848
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