Reply to “PDLIM5 is not a neuronal CaV2.2 adaptor protein”

Maeno-Hikichi et al. reply:

Our original conclusion that the PKC binding protein, enigma homolog (ENH), interacts specifically with both PKCe and N-type Ca²⁺ channels, forming a PKCε-ENH–Ca²⁺ channel macromolecular complex was based on a polyclonal antibody that used the last 18 amino acids of ENH as an epitope. We do not have any more stock of this original antibody. The Abnova antibody used by Stanley and colleagues was raised against the entire ENH protein (a total of 597 amino acids) and this may explain some of the differences between our results. However, the antibody that we supplied to Stanley and colleagues was an antibody that was similar to the one that we used in our original study and was also raised against the last 18 amino acids of ENH. We are not sure why Stanley and colleagues found this antibody to be nonspecific. In addition, it is not clear to us why inclusion of a fusion protein containing ENH binding domains in their recording pipette did not facilitate modulation of N-type Ca²⁺ channel activity by PKC in the recorded rat DRG neurons.