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A time-less function for mouse Timeless

Nature Neuroscience volume 3, pages 755–756 (2000)Cite this article

Abstract

The timeless (tim) gene is essential for circadian clock function in Drosophila melanogaster1,2. A putative mouse homolog, mTimeless (mTim), has been difficult to place in the circadian clock of mammals. Here we show that mTim is essential for embryonic development, but does not have substantiated circadian function.

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Figure 1: Mouse TIM does not inhibit CLOCK/MOP4:BMAL1-mediated transcription in S2 cells.
Figure 2: Phylogenetic analysis of TIM-related sequences.
Figure 3: Targeted mutagenesis of the murine Timeless gene arrests peri-implantational development.

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Acknowledgements

We thank Lauren Shearman for assistance with in situ hybridization experiments. Supported by National Institutes of Health grants R37 HD14427 and RO1 NS39303. A.L.G. was supported by NRSA MH12117.

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Authors and Affiliations

  1. Laboratory of Developmental Chronobiology, Massachusetts General Hospital,, Boston, 02114 , Massachusetts, USA

    Anthony L. Gotter, David R. Weaver, Sathyanarayanan Sriram & Steven M. Reppert

  2. Department of Surgery, Pediatric Surgical Research Laboratory, Massachusetts General Hospital, Boston, 02114, Massachusetts, USA

    Thomas Manganaro & David T. MacLaughlin

  3. Department of Pharmacology, University of Texas Health Science Center in San Antonio, San Antonio, 78284-7764, Texas, USA

    Lee F. Kolakowski Jr.

  4. Department of Biology, Skidmore College, Saratoga Springs, New York, 12866, USA

    Bernard Possidente

Authors
  1. Anthony L. Gotter
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  2. Thomas Manganaro
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  3. David R. Weaver
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  4. Lee F. Kolakowski Jr.
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  5. Bernard Possidente
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  6. Sathyanarayanan Sriram
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  7. David T. MacLaughlin
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  8. Steven M. Reppert
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Correspondence to Steven M. Reppert.

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Gotter, A., Manganaro, T., Weaver, D. et al. A time-less function for mouse Timeless. Nat Neurosci 3, 755–756 (2000). https://doi.org/10.1038/77653

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