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Growth cone and dendrite dynamics in zebrafish embryos: early events in synaptogenesis imaged in vivo

Nature Neurosciencevolume 3pages231237 (2000) | Download Citation

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Abstract

We used time-lapse fluorescence microscopy to observe the growth of Mauthner cell axons and their postsynaptic targets, the primary motor neurons, in spinal cords of developing zebrafish embryos. Upon reaching successive motor neurons, the Mauthner growth cone paused briefly before continuing along its path. Varicosities formed at regular intervals and were preferentially associated with the target regions of the primary motor neurons. In addition, the postsynaptic motor neurons showed highly dynamic filopodia, which transiently interacted with both the growth cone and the axon. Both Mauthner cell and motor neurons were highly active, each showing motility sufficient to initiate synaptogenesis.

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Acknowledgements

Susan Pike conducted initial work on this project. We thank S. Pike and faculty members of the MBL Neural Development and Genetics of the Zebrafish course (Woods Hole, Massachusetts) for help and advice. J.D.J. is a fellow of the Helen Hay Whitney Foundation. This work was supported by NIH grants to S.J.S.

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  1. Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, 94305-5435, California, USA

    • James D. Jontes
    • , JoAnn Buchanan
    •  & Stephen J. Smith

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Correspondence to Stephen J. Smith.

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https://doi.org/10.1038/72936

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