Supplementary Figure 1 : MADM labeling of thalamic clones.

From: Ontogenetic establishment of order-specific nuclear organization in the mammalian thalamus

Supplementary Figure 1

(a) Confocal images of an E12 Nestin-CreERT2;Ai9-tdTomato brain treated with TM at E10 and stained for BLBP (green), a radial glial progenitor-specific marker, tdTomato (red), and OLIG3 (white), and with DAPI (blue). A schematic of brain structures is shown at the top. Note that BLBP-expressing radial glial progenitors in the developing thalamus marked by OLIG3 expression are labeled by tdTomato upon TM treatment, indicating that CreERT2 is expressed in thalamic radial glial progenitors in the Nestin-CreERT2 mouse line. Scale bar: 100 μm. (b) Schematic of MADM labeling. (c) Experimental paradigm of MADM-based clonal analysis. (d) Confocal images of an E12 brain treated with TM at E10 and stained for EGFP (green) and tdTomato (red), and with DAPI (blue). High magnification image of a G2-Z yellow clone in the thalamus (boxed area) is shown to the right. Arrows indicate bipolar radial glial progenitors, arrowheads indicate the progeny, open arrowheads indicate the radial glial fibers, and the asterisk indicates the ventricular endfoot. Scale bars: 100 μm and 20 μm.