Supplementary Figure 12 : Shh signaling affects gliogenesis in the thalamus.

From: Ontogenetic establishment of order-specific nuclear organization in the mammalian thalamus

Supplementary Figure 12

(a) Quantification of the average number of glial cells in individual clones in the control (left) and SmoM2 (right) thalami (control, n=34; SmoM2, n=24). Data are presented as median with interquartile range, and whiskers are the minimum and maximum. n.s., not significant (Mann Whitney test). (b) Quantification of the number of clones containing neurons only (N) or neurons and glia (N+G) in the wild type control (left) and SmoM2 (right) thalami. Note the increase in the fraction of clones containing glial cells in the SmoM2 thalamus compared to the control. **, p=0.003 (χ2 test). (c) NND analysis of MADM-labeled glial clones in the SmoM2 thalamus. Note that compared to random datasets simulated 100 times (gray), the cumulative frequency of NND of thalamic glial clones (blue, n=14) is significantly left-shifted towards shorter distances, indicating a spatial clustering. Data are presented as mean±s.e.m. ****, p=1x10–15 (unpaired t test with Welch’s correction). (d) Schematic representation of lineage-related nuclear formation and functional organization in the thalamus.