Supplementary Figure 10 : Expression of SmoM2-EYFP in MADM-labeled thalamic clones.

From: Ontogenetic establishment of order-specific nuclear organization in the mammalian thalamus

Supplementary Figure 10

Representative confocal images of a G2-X clone labeled at E10, and immunostained for EYFP (green) and tdTomato (red), and counterstained with DAPI (blue) at E14. High magnification images of a tdTomato-expressing RGP in the VZ (broken lines) are shown to the right. Note the membrane expression of SmoM2-EYFP in the tdTomato-expressing RGP (arrows) with an apical endfoot (asterisks), suggesting the successful recombination of both SmoM2-EYFP and MADM11 alleles in the clone. Arrowheads indicate the expression of SmoM2-EYFP in a nearby non-MADM-labeled cell, indicating a higher frequency of intra-chromosomal (i.e. SmoM2-EYFP allele) recombination than that of inter-chromosomal (i.e. MADM11 allele) recombination. Broken line circles indicate the somas. Scale bars: 20 μm and 10 μm.