Supplementary Figure 6 : Inhibitory interneuron clones in TRN.

From: Ontogenetic establishment of order-specific nuclear organization in the mammalian thalamus

Supplementary Figure 6

(a) 3D reconstructed images of a G2-X clone containing 7 labeled neurons in the TRN contoured by yellow lines. (b) High magnification cross-section confocal images of the 7 labeled neurons stained for parvalbumin (PV, white), a GABAergic interneuron-specific marker. Note that all 7 neurons are positive for PV, indicating their interneuron identity. Scale bar: 10 μm. (c) Quantification of the average number of neurons in the four clonal clusters ('mvp', n=33; 'a', n=9; 'md', n=18; 'GABAergic', n=17). Data are presented as median with interquartile range, and whiskers are the minimum and maximum. **, p=0.005 (‘mvp’ versus ‘a’, MannWhitney test); **, p=0.001 (‘mvp’ versus ‘GABAergic’, Mann-Whitney test). (d) Quantification of the average number of nuclei that the four clonal clusters occupy. Data are presented as median with interquartile range, and whiskers are the minimum and maximum. n.s., not significant; ****, p<0.0001 (Mann Whitney test. Details are available in the Supplementary Methods Checklist).