Supplementary Figure 4 : Distinct morphological features of excitatory neurons, astrocytes, and oligodendrocytes in the thalamus.

From: Ontogenetic establishment of order-specific nuclear organization in the mammalian thalamus

Supplementary Figure 4

(a, b) Representative 3D projection (a) and confocal (b) images of a G2-X clone (green and red) in the thalamus labeled at E9, and immunostained for neuronal marker NEUN (white) and counterstained with DAPI (blue) at P21. High magnification images of the somas of four cells are shown to the right in b. Broken line circles indicate the nuclei. Scale bars: 20 μm and 20 μm. (c, d) Representative 3D projection (c) and confocal (d) images of a G2-X thalamic clone (green and red) occupying TRN (contoured in yellow lines) and ZI labeled at E9, and immunostained for astrocyte marker S100 (white), and counterstained with DAPI (blue) at P21. High magnification images of the soma of the astrocyte are shown to the right in d. The broken line circle indicates the nucleus. Scale bars: 20 μm and 20 μm. (e, f) Representative 3D projection (e) and confocal (f) images of a G2-Z thalamic clone (yellow) occupying TRN (contoured in yellow lines) and ZI labeled at E9, and immunostained for oligodendrocyte marker OLIG2 (white), and counterstained with DAPI (blue) at P21. High magnification images of the somas of two oligodendrocytes (arrows) are shown to the right in e. Broken line circles indicate the nuclei. Note the labeled oligodendrocytes possess characteristic short parallel neurites. Scale bars: 20 μm and 20 μm. (g) Quantification of the fraction of labeled cells with a large soma/nucleus and long neurites or a small soma/nucleus and short bushy or parallel neurites that are positive for NEUN, S100, or OLIG2, respectively.