Neural networks that control reproduction must integrate social and hormonal signals, tune motivation, and coordinate social interactions. However, the neural circuit mechanisms for these processes remain unresolved. The medial preoptic area (mPOA), an essential node for social behaviors, comprises molecularly diverse neurons with widespread projections. Here we identify a steroid-responsive subset of neurotensin (Nts)-expressing mPOA neurons that interface with the ventral tegmental area (VTA) to form a socially engaged reward circuit. Using in vivo two-photon imaging in female mice, we show that mPOANts neurons preferentially encode attractive male cues compared to nonsocial appetitive stimuli. Ovarian hormone signals regulate both the physiological and cue-encoding properties of these cells. Furthermore, optogenetic stimulation of mPOANts–VTA circuitry promotes rewarding phenotypes, social approach and striatal dopamine release. Collectively, these data demonstrate that steroid-sensitive mPOA neurons encode ethologically relevant stimuli and co-opt midbrain reward circuits to promote prosocial behaviors critical for species survival.
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We thank the Stuber laboratory for discussion and support. We thank S. Smith for advice on two-photon imaging, the UNC vector core for viral packaging, and the UNC Neuroscience Center Microscopy Core, especially V. Ghukasyan, for his training and assistance (P30 NS045892). We thank K. Deisseroth for viral constructs (Stanford University). We thank L. Tarantino and S. Schoenrock for their input and assistance with hormone manipulation procedures. We thank K. Schilling-Scrivo, L. Eckman, J. Rodriguez and G. Tipton for technical assistance and R. Ung for assistance with coding. J.A.M. was supported by the National Institute of Mental Health (T32-MH093315), J.M.O. was supported by the National Institute on Drug Abuse (F32-DA041184), Z.A.M. was supported by ABMRF and NIAAA (K01 AA020911), J.E.R. was supported by NIAAA (F30AA021312) and M.A.R. was supported by NIDDK (F32-DK112564). This study was supported by funds from the Foundation of Hope, the Brain and Behavior Research Foundation, the Simons Foundation, the National Institute on Drug Abuse (R01 DA032750 and R01 DA038168) (G.D.S.), the Department of Psychiatry at UNC-CH, and the National Institute on Alcohol Abuse and Alcoholism (AA022449) (E.A.B.).
The authors declare no competing financial interests.
Integrated supplementary information
a. Schematic illustrates Retrobead tracer injection into the VTA and DIO-eYFP injection into the mPOA. VTA: ventral tegmental area, mPOA: medial preoptic area.b. Confocal image of Retrobead injections into the VTA from a representative animal. PAG: periaqueductal gray, D: dorsal, V: ventral, M: medial, L: lateral, scale bar: 100 μm.c. Confocal image of Nts Ai9 reporter expression (blue) and ESR1-ir (pink). ESR1: estrogen receptor 1.d. Confocal image of Nts labeled neurons and nuclear ESR1-ir in the mPOA scale bar: 40 μm.
a. Confocal image of Nts (green) and Vgat (blue) mRNA in the mPOA (left). Scale bar: 200 μm. Color dot plot from a representative brain slice illustrates the X-Y coordinates of each Vgat expressing cell that is also positive (green) or negative (blue) for Nts (right hemisphere). Scale bar: 500 μm, Nts: neurotensin, Vgat: vesicular GABA transporter, mPOA: medial preoptic area, vBNST: ventral bed nucleus of the stria terminalis, D: dorsal, V: ventral, M: medial, L: lateral, AC: anterior commissure, OX: optic chiasm.b. Confocal image of Nts (green) and Vglut2 (pink) mRNA in the mPOA (left). Scale bar, 200 μm. Color dot plot from a representative subject illustrates the X-Y coordinates of each Vglut2 expressing cell that is positive (green) or negative (pink) for Nts. Scale bar, 500 μm, Vglut2: vesicular glutamate transporter, for remaining abbreviations refer above (A).c. Confocal image of Nts (green), Vgat mRNA (blue), and Vglut2 mRNA (pink) mRNA in the mPOA. Scale bar: 20 μm.d. Cumulative distribution plot showing that the mean intensity of Nts expression within each cell that is positive for Nts is higher in estradiol treated mice compared to vehicle treated controls (Kolmogorov-Smirnov D = 0.53, p < 0.0001, 1,900 cells, n = 3 mice per group).e. Cumulative distribution plot showing that the mean intensity of Vgat expression within each cell that is positive for Nts is higher in estradiol treated mice compared to vehicle treated controls (Kolmogorov-Smirnov D = 0.35, p < 0.0001, 1,900 cells, n = 3 mice per group).f. Cumulative distribution plot showing that the mean intensity of Vgat expression within each cell that is negative for Nts is higher in estradiol treated mice compared to vehicle treated controls (Kolmogorov-Smirnov D = 0.11, p < 0.0001, 1,900 cells, n = 3 mice per group).g. Cumulative distribution plot showing that the mean intensity of Vglut2 expression within each cell that is negative for Nts is higher in estradiol treated mice compared to vehicle treated controls (Kolmogorov-Smirnov D = 0.23, p < 0.0001, n = 3 mice per group).h. Pie chart illustrates the percentage of Nts neurons co-expressing Vgat and/or Vglut2.
a. Representative confocal image of Nts mRNA (green) in the mPOA. mPOA: medial preoptic area, vBNST: ventral bed nucleus of the stria terminalis, OX: optic chiasm, scale bar: 200 μm.b. Representative confocal image of Cre mRNA (magenta) in the mPOA.c. Merged confocal image of Nts mRNA (green) and Cre mRNA (magenta).d. Representative confocal image of Nts mRNA (green) and Cre mRNA (magenta) in the mPOA. Scale bar: 40 μm.e. Quantification of Nts mRNA (green) and Cre mRNA (magenta) overlap in the mPOA. Pie chart represents overlap.
a. Schematic represents whole cell patch clamp recordings in an mPOANts::GCaMP6 neuron while simultaneously imaging Ca2+ activity. Representative cell shows that increasing current injection increased the intensity of Ca2+ fluorescence.b. Example calcium traces, F/F0 during patch clamp recordings in mPOANts::GCaMP6 cells from ovariectomized mice treated with vehicle (vehicle) or estradiol (E2) primed 48 hours and 4 hours prior to collecting slices.c. Estradiol enhanced the Z-score of normalized peak response from baseline in mPOANts::GCaMP6 cells, compared to controls, and increasing current injection increased z-score. (Two-Way ANOVA, interaction F8,104 = 4.47, p < 0.0001, Veh n= 6 cells, E2 n= 9 cells; 2 mice per group).d. Schematic depicts surgical and in vivo imaging approach. Cre-inducible GCaMP6s in mPOANts neurons and Gradient Refractive Index (GRIN) lens for two-photon optical access in awake head-fixed mice.e. Image represents a standard deviation z-projection acquired from a motion corrected two-photon baseline recording from mPOANts::GCaMP6. Images were used for hand drawing regions of interests (ROIs) over cells.f. Illustration is an overlay representing hand drawn regions of interest (ROIs) onto a standard deviation projection (c) for subsequent somatic Ca2+ signal extraction in individual neurons.
a. Schematic illustrates test for behavioral odor preference for an odor following a habituation to an empty arena.b. Female mice had no side preference during habituation periods that occurred prior to odor testing (paired t-test, t1,9 = 1.09 p = 0.3061, n = 10 mice).c. Nose-point time was highest during odor trials consisting of intact male urine, compared to all other odorants. (One-way Anova, F4,37 = 10.60 p < 0.0001, n = 7-10 mice).d. There was no difference in the amount of nose-point time in the saline control zone during each odor test. (One-way Anova, F4,37 = 1.05 p = 0.3950, n = 7-10 mice).e. All odors induced a behavioral preference for the odor zone over the saline control zone and the preference index for male urine was highest than that of gonadectomzied male or female urine. (One-way Anova, F4,37 = 5.38, p = 0.0016, n = 7-10 mice).f. Females had the highest odor zone approach bouts to male urine. (One-way Anova, F4,37 = 3.34, p = 0.0196, n = 7-10 mice).g. Females did not display differences in saline approach bouts. (One-way Anova, F4,37 = 2.01, p = 0.1131, n = 7-10 mice).
a. Schematic illustrates steroid-priming of estradiol (E2) and subsequently progesterone (P4) 4-6 hours prior to behavioral odor testing and compared with an oil injection (vehicle).b. Heat maps of total time spent in an odor zone from a representative female before and after estradiol priming.c. Ovariectomzied females had a higher preference for male odor following estradiol priming that was sustained following progesterone administration, with no differences between steroid treatments (One-way Anova, F2,17 = 35.61, p = 0.0003, n = 6 mice). Veh. vs. E2, **P = 0.004; Veh. vs. P4, ***P = 0.0003.d. Ovariectomzied females spent more time in the odor zone following estradiol priming and this was sustained following progesterone administration, with no differences between steroid treatments (Oneway Anova, F2,17 = 27.86, p = 0.0008, n = 6 mice). Veh. vs. E2, **P = 0.009; Veh. vs. P4, ***P = 0.00019.e. No treatment differences were detected in the amount of time spent investigating the control block. (One-way Anova, F2,17 = 1.76, p = 0.238, n = 6 mice).f. Ca2+ signal normalized to baseline and averaged across male trials. Heat map illustrates response from the same 230 mPOANts::GCaMP6 cells across three separate imaging days. Cells are sorted by male odor response on the day of E2 administration (middle heat plot) and indexed in the same order for comparison on the day of Veh administration (left heat plot) and after P4 administration (right heat plot).g. Top: Pie charts illustrate the percentage of cells excited (green) or inhibited (blue) in response to male odor by treatment. Bottom: Averaged Ca2+ traces from mPOANts::GCaMP6 cells significantly excited by male odor after estradiol, compared to their response after vehicle or progesterone (Wilcoxon t-test, all p values < 0.05, n = 230 cells combined across 4 mice).h. Ca2+ signal normalized to baseline and averaged across female trials. Heat map illustrates response from the same 230 mPOANts::GCaMP6 cells across two separate imaging days. Cells are sorted by female odor response on the day of estradiol administration (right heat plot) and indexed in the same order for comparison on the day of Veh administration (left heat plot).i. mPOANts::GCaMP6 cells imaged across 2 days of the estrous cycle. Arrows refer to examples of the same cells.j. Example Ca2+ traces, F/F0, of GCaMP6s Ca2+ dynamics from mPOANts::GCaMP6 cells across two days of the estrous cycle. Arrows point out examples of the same cells across days.k. mPOANts::GCaMP6 cells had Ca2+ events longer in duration on proestrus compared to estrus (Paired t-test, t154= 2.26, p = 0.0255, n = 155 cells combined across 3 mice).l. mPOANts::GCaMP6 cells had calcium events higher in peak amplitude on proestrus compared to estrus (Paired t-test, t154= 2.56, p = 0.0113, n = 155 cells combined across 3 mice.
a. Estrous cycle cytology representing the four days of the mouse estrous cycle. P: proestrus, E: estrus, DI: metestrus, DII: diestrus, scale bar, 62 μm.b. Individual number of nose pokes into the active port from mPOANts::ChR2 females during proestrus and estrus. Regardless of cycle, differences were observed in the number of pokes as a function of frequency, with differences detected between all frequencies except for between 5 and 10 Hz (Two-Way ANOVA, frequency, F3,18 = 199, p < 0.0001, ChR2 n = 4 mice).c. mPOANts::ChR2 females poked more during 20 and 40 Hz (but not 5 or 10 Hz) of photostimulation on proestrous compared to estrous. (Two-Way ANOVA, interaction, F3,18 = 14.10, p < 0.0001, ChR2 n = 4 mice).d. mPOANts::ChR2 females displayed a significantly higher mean velocity in the stimulation side of the chamber in all phases of the estrous cycle compared to mPOANts::eYFP controls (Two-Way ANOVA, interaction F3,30 = 5.75, p = 0.0031, eYFP n = 7 mice, ChR2 n = 5 mice).e. mPOANts::ChR2 males displayed a significantly higher mean velocity on the stimulation side of the chamber compared to mPOANts::eYFP controls with no effect of day (Two-Way ANOVA, group effect, F1,8 = 31.10, p = 0.0008, eYFP n = 6 mice, Chr2 n = 4 mice).f. Location of optical fiber placements in the mPOA from mPOANts male and female optogenetic cohorts.
a. Confocal image from an mPOANts::ChR2 mouse (top) and mPOANts::eYFP control (bottom). vBNST= ventral bed nucleus of the stria terminalis. D: dorsal, V: ventral, M: medial, L: lateral, AC: anterior commissure, OX: optic chiasm, scale bar: 200 um.b. Confocal image of mPOANts::ChR2 (green) and optically-evoked Fos immunoreactivity (red), scale bar: 50 um (left column).c. 20 Hz photostimulation increased the number of cells expressing Fos immunoreactivity in mPOANts::ChR2 mice compared to mPOANts::eYFP controls (Unpaired t-test t4 = 15.87, p < 0.0001, n = 3 mice per group).
Supplementary Figure 9 Optogenetic modulation of mPOANts neurons during hormonal and social contexts.
a. Top: Schematic illustrates testing paradigm. Females were tested for real-time place preference after a vehicle control injection once a week preceding and following steroid priming. Steroid-priming consisted of an injection of estradiol and progesterone 48 hours apart, with testing 4 hours after each injection.Bottom: Stimulation of mPOANts::ChR2 neurons increased the amount of time spent in the photostimulation side of the chamber and steroid priming resulted in an amplified increase in real-time preference for weeks following hormone treatment, compared to those treated with vehicle and mPOANts::eYFP controls (Two-Way ANOVA, interaction F10,75 = 8.91 p < 0.0001, n = 5 – 7 mice per group). E2: estradiol, Veh: vehicle.b. Photostimulation increased the amount of time mPOANts::ChR2-eYFP males spent in the female zone (Two-Way ANOVA, Interaction F1,8 = 15.20, p = 0.0046, eYFP n= 6, ChR2 n = 5).c. Photostimulation did not affect the amount of time mPOANts::ChR2-eYFP males spent in the male zone (Two-Way ANOVA, Interaction F1,8 = 5.21, p = 0.0564, eYFP n= 6, ChR2 n = 5).d. Photostimulation enhanced female preference in mPOANts::ChR2-eYFP males (Two-Way ANOVA, Interaction F1,8 = 15.80, p = 0.0033, eYFP n= 6, ChR2 n = 5).e. Mating experience in conjunction with steroid priming enhanced male preference (Two-Way ANOVA, Group F5,35 = 3.68, p = 0.0088, n = 7 mice per group).f. Photoinhibition did not affect amount of time spent in the female zone (Two-Way ANOVA, Group F1,12 = 0.045, p = 0.8353, n = 7 mice per group).g. Photoinhibition did not affect real-time place aversion in mPOANts::NpHR females or mPOANts::eYFP controls across vehicle or steroid-primed conditions (Two-Way ANOVA, interaction, F2,16 = 2.52, p = 0.1118, n = 7 mice per group).h. Schematic illustrates chronic fiber placements in the mPOA from ChR2 and NpHR optogenetic behavioral cohorts and placements in the VTA from ChR2 mice.
a. Schematic illustrates two-chamber assay with a control zone (empty cup) and a food zone (high-fat diet cup). Light stimulation was delivered in alternating 10 min time epochs.Color heat maps illustrating the spatial location in the food preference assay for a representative previously-primed mPOANts::eYFP control mouse.Color heat maps illustrate the spatial location in the food preference assay for a representative previously-primed mPOANts::ChR2 mouse.b. No group by time interaction was detected in the amount of time spent in the food zone mice (Two-Way ANOVA, interaction F4,26 = 1.04 p = 0.4044, n = 5-6 mice per group).c. No group by time interaction was detected in the amount of time spent in the food zone mice (Two-Way ANOVA, interaction F4,26 = 0.21 p =.09322 n = 5-6 mice per group).d. Optogenetic stimulation increased the number of food-zone approach bouts in previously-primed mPOANts::ChR2 females compared to vehicle-treated mPOANts::ChR2 and previously-primed eYFP control mice. (Two-Way ANOVA, interaction F4,26 = 6.51 p = 0.0009, n = 5-6 mice per group).e. Optogenetic stimulation did not affect the number of control cup approach bouts and this did not differ by group. (Two-Way ANOVA, interaction F4,26 = 0.24 p = 0.9122, n = 5-6 mice per group).f. No group differences of light stimulation were detected in time preference spent in the food zone over the control zone (One-Way ANOVA, interaction F2,13 = 0.44 p = 0.6531, n = 5-6 mice per group).g. Optogenetic stimulation increased the rate per minute that previously-primed mPOANts::ChR2 females made direct contact with the food compared to vehicle-treated mPOANts::ChR2 and previously-primed eYFP control mice (Two-Way ANOVA, interaction F2,15 = 7.51 p = 0.0055, n = 5-6 mice per group).h. Optogenetic stimulation did not affect the amount of time spent eating. (Two-Way ANOVA, interaction F4,26 = 2.14 p = 0.1050, n = 5-6 mice per group).i. Optogenetic stimulation did not affect food consumption. (One-Way ANOVA, interaction F2,17 = 0.15 p = 0.8640, n = 6 mice per group).j. Optogenetic inhibition did not affect the amount of time spent in the food zone. (Two-Way ANOVA ANOVA, interaction F2,22 = 0.47 p = 0.9543, n = 6-7 mice per group).k. Optogenetic inhibition did not affect the amount of time spent in the control zone. (One-Way ANOVA, interaction F2,22 = 0.74 p = 0.4868, n = 6-7 mice per group).l. Optogenetic inhibition did not affect preference for the food zone (One-Way ANOVA, interaction F1,11 = 3.82, p = 0.0766, n = 6-7 mice per group).m. Optogenetic inhibition did not affect the number of visits into food zone. Two-Way ANOVA ANOVA, interaction F2,22 = 0.69 p = 0.5142, n = 6-7 mice per group).n. Optogenetic inhibition did not affect the number of visits into the control zone. (Two-Way ANOVA, interaction F2,22 = 0.405 p = 0.6719, n = 6-7 mice per group).o. Optogenetic inhibition did not affect the amount of high fat diet consumption. (un-paired t-test, t1,10 = 0.70 p = 0.4983, n = 6 mice).
Supplementary Figures 1–10 (PDF 4665 kb)
Representative video of spontaneous baseline Ca2+ dynamics in mPOANts::GCaMP6s neurons during in vivo two-photon imaging (2p) of an awake head-fixed female in proestrus. Imaging was acquired at 5 Hz but sped up ×4. (MP4 18928 kb)
Representative video of Ca2+ dynamics in mPOANts::GCaMP6s neurons during in vivo two-photon imaging in an awake head-fixed female in proestrus. Imaging was acquired at 5 Hz but sped up ×4. Two concatenated odor trials are shown. Male odor was delivered at ~3–5 s and ~13–15 s. (MP4 4372 kb)
Representative video of a mPOANts::ChR2 female in proestrus during optical self-stimulation test. In this video the female nose-pokes through an active port on the left to receive 20 Hz photostimulation with a 5 ms pulse width and for a duration of 3 s. (MP4 10924 kb)
Representative video of a mPOANts::ChR2 female in proestrus during a real-time place preference optogenetic behavioral assay. In this video then female received 20 Hz photostimulation only in the right side of the arena. (MP4 17107 kb)
Representative video of a mPOANts::ChR2 ovariectomized female that was previously steroid-primed. In this video the female is being photostimulated at 20 Hz during a social preference assay with two stimulus mice in the holding chambers: an adult male in the left chamber and an ovariectomized adult female on the right chamber. (MP4 15018 kb)
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McHenry, J., Otis, J., Rossi, M. et al. Hormonal gain control of a medial preoptic area social reward circuit. Nat Neurosci 20, 449–458 (2017). https://doi.org/10.1038/nn.4487
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