Supplementary Figure 3: REM sleep deprivation by gentle handling reduces the duration of REM sleep over 8–24 h in P30 mice. | Nature Neuroscience

Supplementary Figure 3: REM sleep deprivation by gentle handling reduces the duration of REM sleep over 8–24 h in P30 mice.

From: REM sleep selectively prunes and maintains new synapses in development and learning

Supplementary Figure 3

(a) The total time of NREM and wakefulness were not significantly affected by REMD over 8-16 h (n = 7, 5, 6 mice for ND, NREM-d, REMD, respectively). (b) The average episode duration of REM sleep was significantly reduced in REMD mice as compared to non-deprived control mice and NREM-d mice between 8-16h (n = 7, 5, 6 mice for ND, NREM-d, REMD, respectively). (c) The episode number of REM sleep were not affected by REMD over 8-16 h (n = 7, 5, 6 mice for ND, NREM-d, REMD, respectively). (d) The total time of NREM and wakefulness were not significantly affected by REMD over 16-24 h (n = 7, 5, 6 mice for ND, NREM-d, REMD, respectively). (e) The average episode duration of REM sleep was significantly reduced in REMD mice as compared to non-deprived control mice and NREM-d mice between 16-24 h (n = 7, 5, 6 mice for ND, NREM-d, REMD, respectively). (f) The episode number of REM sleep were not affected by REMD over 16-24h (n = 7, 5, 6 mice for ND, NREM-d, REMD, respectively). (g) The number of gentle handling for NREM-d and REMD were comparable between NREM-d and REMD mice over 8-24h (n = 5, 6 mice for NREM-d, REMD). Data are presented as mean ± s.e.m. **P < 0.01, n.s. = not significant, Wilcoxon–Mann–Whitney test.

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