Supplementary Figure 2: REM sleep deprivation by gentle handling reduces the duration of REM sleep over 8–16 h in P21 mice. | Nature Neuroscience

Supplementary Figure 2: REM sleep deprivation by gentle handling reduces the duration of REM sleep over 8–16 h in P21 mice.

From: REM sleep selectively prunes and maintains new synapses in development and learning

Supplementary Figure 2

(a) The total time of NREM and wakefulness were not significantly affected by REMD (n = 4, 4, 5 mice for ND, NREM-d, REMD, respectively). (b) The average episode duration of REM sleep was significantly reduced in REMD mice as compared to non-deprived control mice and NREM-d mice between 8-16 h (n = 4, 4, 5 mice for ND, NREM-d, REMD, respectively). (c) The episode number of REM sleep were not significantly affected by REMD (n = 4, 4, 5 mice for ND, NREM-d, REMD, respectively). (d) The number of gentle handling for REMD and NREM-d in P21 mice. To control for non-specific effect of gentle handling, the number of gentle handling for REMD was recorded each hour in REMD mice (n = 5 mice). The comparable number of gentle handling was applied to NREM-d control mice during NREM sleep (n = 4 mice). Data are presented as mean ± s.e.m. *P < 0.05, n.s. = not significant, Wilcoxon–Mann–Whitney test.

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