Supplementary Figure 1 : Methodological considerations for and quality control of single-cell RNA-seq

From: Molecular interrogation of hypothalamic organization reveals distinct dopamine neuronal subtypes

Supplementary Figure 1

(a) Neuron numbers per cluster in our analysis (blue boxes), and comparison of actual numbers in repeat experiments with statistical probing of random distribution (solid red circles). Note that this comparison excluded sampling or processing-related bias due either to false positive observations or undersampling. (b) Bar plots show the total number of genes detected in individual neuronal subtypes. (c) Likewise, the total number of mRNA molecules passing our filtering criteria (see Online Methods) were plotted. Grey circles and error bars represent means ± s.e.m. per group in (b,c).