Supplementary Figure 2: rAAV-mDlx-GFP is not selective for interneurons in the spinal cord | Nature Neuroscience

Supplementary Figure 2: rAAV-mDlx-GFP is not selective for interneurons in the spinal cord

From: A viral strategy for targeting and manipulating interneurons across vertebrate species

Supplementary Figure 2

(a, b) P0 wildtype Swiss Webster mice (n=10) were injected with the indicated virus in the lumbar spinal cord and analyzed by immunostaining for GFP and PAX2 immunoreactivity after one weeks. Representative example of expression of the reporter GFP and Pax2 and corresponding quantifications. (Upper panel: 2.9 +/- 1.7%, n= 255 cells from 4 animals; lower panel: 12.2 +/- 1.1%, n= 1904 cells from 6 animals). Quantification are indicated in the text as mean ± s.e.m and are represented as box-and-whisker plot with upper and lower whiskers represent the maximum and minimum value respectively and the box represent upper, median and lower quartile. Dashed lines represent limits of the spinal cord. Scale bars represent 25μm (left merge) 100μm (right merge).

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