Adult Zebra Finches (n=4) were injected with rAAV-mDlx-GFP in HVC and were either sectioned for electrophysiological recording after 2 weeks or a cranial window was placed above the injection site for two-photon imaging and in-vivo electrophysiological recording. (a, b) Representative examples of biocytin GFP positive cells recorded from acute slice showing typical neocortical interneuron morphology and corresponding membrane potential of cells in response to current pulses. (c) Representative examples of biocytin GFP positive recorded in vivo and membrane potential of the two cells marked with stars in response to current pulses. Dark gray: hyperpolarizing; Black trace: rheobase; light gray: -200pA / twice rheobase. Scale bars represent 20μm (a, b) or 10μm (c).