Supplementary Figure 2: CSF1 is induced in injured sensory neurons and co-transported with NPY to the spinal cord, where CSF1R is expressed in microglia. | Nature Neuroscience

Supplementary Figure 2: CSF1 is induced in injured sensory neurons and co-transported with NPY to the spinal cord, where CSF1R is expressed in microglia.

From: Injured sensory neuron–derived CSF1 induces microglial proliferation and DAP12-dependent pain

Supplementary Figure 2

(a) CSF1 induction in DRG neurons ipsilateral to the nerve injury co-localizes with NPY (Inset), a neuropeptide that is only expressed in sensory neurons after injury (1 d). Scale bars represent 200 μm and 10 μm (Inset); (b) Accumulation of CSF1 and NPY at the dorsal root ligature (4d post injury). Co-localization of CSF1 with NPY establishes that the CSF1 transport is intra-axonal. Dashed line denotes ligature. Scale bar represents 200 μm; (c) CSF1R (immunostaining) co-localizes with the microglial marker CD11b and both markers are induced in the dorsal horn after nerve injury (3 d post injury). Scale bar represents 100 μm; (d) There is complete CSF1R-GFP co-localization with the microglial marker Iba1, and none with the neuronal marker, NeuN. Both CSF1R-GFP and Iba1 are induced in the dorsal horn after nerve injury (3 d post injury). White square shows enlarged region. Scale bar represents 100 μm; (e) Quantification of CSF1R immunostaining in CD11b positive cells in the superficial dorsal horn 3 days after nerve injury; (f) Quantification of GFP intensity in Iba1 positive cells in the superficial dorsal horn 3 days after nerve injury. N = 3–4 mice/group. Data are presented as mean ± s.e.m. *p≤0.05, ***p≤0.001.

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