Neuronal activity is important for the functional refinement of neuronal circuits in the early visual system. At the level of the cerebral cortex, however, it is still unknown whether the formation of fundamental functions such as orientation selectivity depends on neuronal activity, as it has been difficult to suppress activity throughout development. Using genetic silencing of cortical activity starting before the formation of orientation selectivity, we found that the orientation selectivity of neurons in the mouse visual cortex formed and matured normally despite a strong suppression of both spontaneous and visually evoked activity throughout development. After the orientation selectivity formed, the distribution of the preferred orientations of neurons was reorganized. We found that this process required spontaneous activity, but not visually evoked activity. Thus, the initial formation and maturation of orientation selectivity is largely independent of neuronal activity, and the initial selectivity is subsequently modified depending on neuronal activity.
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We thank T. Yokomizo (Juntendo University) for help and advice on molecular biology works, such as plasmids construction, Y. Tezuka (Kyoto University) for sharing the information about Tet-gene expression system development and histology works, M.H. Histed (Harvard Medical School) for reading the manuscript, K. Kitamura (University of Yamanashi), M. Kano and A. Takeuchi (University of Tokyo) for showing cell-attached recordings, S. Kondo (Kyushu University) for electrophysiology set up, K. Hayashi (Kyushu University) for plasmids construction, A. Honda and Y. Sono (Kyushu University) for animal care and genotyping, T. Hirano (Kyoto University), all of the members of Ohki laboratory for support and discussion, and the Research Support Center, Graduate School of Medical Sciences, Kyushu University for technical support. This work was supported by grants from CREST-JST (to K.O. and Y.T.), JSPS KAKENHI (grant number 25221001 to K.O., 23500388 to Y.T.), JST, Strategic International Research Cooperative Program, SCIP (to K.O.), Grant-in-Aid for Scientific Research on Innovative Areas, “Glial assembly: a new regulatory machinery of brain function and disorders” (25117004 to K.O.) and “Neural Diversity and Neocortical Organization” (23123508 and 25123707 to Y.T.), grants from the Ichiro Kanehara Foundation for the Promotion of Medical Sciences and Medical Care, and the Uehara Memorial Foundation (to T.Y.). A part of this work was carried out under the Brain/MINDS by the MEXT of Japan. K.M.H. was supported by Takeda Science Foundation. T. M. was supported by JSPS Research Fellowship for Young Scientists(201503597).
The authors declare no competing financial interests.
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Hagihara, K., Murakami, T., Yoshida, T. et al. Neuronal activity is not required for the initial formation and maturation of visual selectivity. Nat Neurosci 18, 1780–1788 (2015). https://doi.org/10.1038/nn.4155
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