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GDNF is not required for catecholaminergic neuron survival in vivo

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Figure 1: Generation of Gdnf floxed mice and catecholamine system analysis after crosses to Nestin-Cre.
Figure 2: Dopamine system analysis after striatal Gdnf reduction by AAV5-Cre injection in adult mice.
Figure 3: Dopamine system analyses after Gdnf reduction in adult mice.


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We thank S. Wiss and K. Rautio for expert technical assistance. J.-O.A. was supported by the Academy of Finland grants 136591, 140983 and 263700, the US National Institutes of Health (NS 0708259) and by the Institute of Biotechnology. M.S. was supported by grants from Sigrid Jusélius Foundation, H. Lundbeck Foundation, M. J. Fox Foundation for Parkinson's Research, Academy of Finland (grant 11186236), US National Institutes of Health (NS 0708259) and the University of Helsinki. T.P.P. was supported by Päivikki and Sakari Sohlberg Foundation. A.B. was supported by grants from the Swedish Research Council. Mouse Behavioural Phenotyping Facility. V.V. is supported by Biocenter Finland. E.C. is supported by the ERC iPlasticity project (322742), Sigrid Jusélius foundation and the Academy of Finland. S.G. was supported by a postdoctoral stipend from the Swedish Brain Foundation (Hjärnfonden). K.V. is supported by a grant from BnM. J.K. was supported by Research Foundation of the University of Helsinki and The Finnish Cultural Foundation.

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J.-O.A. designed and generated the GDNF conditional knockout mice, K.V., C.V., J.-O.A. and M.-A.H. performed qPCR measurements, J.-O.A. and M.-A.H. performed GDNF ELISA, J.K. and C.V. performed immunohistochemistry and stereology, J.K., C.V. and S.G. dissected the brains, J.K. measured dopamine levels and optical density, S.G. carried out AAV-Cre injections, C.V. did tamoxifen injections, J.L., C.V. and S.G. performed all of the behavioral assays, J.-O.A., M.S., T.P.P., A.B., V.V. and E.C. planned the experiments, M.S., J.-O.A., T.P.P. and A.B. provided funding, and J.K. and J.-O.A. prepared the figures and wrote the paper.

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Correspondence to Jaan-Olle Andressoo.

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Kopra, J., Vilenius, C., Grealish, S. et al. GDNF is not required for catecholaminergic neuron survival in vivo. Nat Neurosci 18, 319–322 (2015).

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