Supplementary Figure 5 : Absence of Fmrp does not alter cortical layering.

From: FMRP regulates multipolar to bipolar transition affecting neuronal migration and cortical circuitry

Supplementary Figure 5

Left, immunohistochemistry showing E17.5 confocal images of coronal sections from WT and Fmr1−/− cortices labeled with anti-COUP-TF-interacting protein 2 antibody (Ctip2, transcription factor) for lower layer neurons and anti-Cut homeobox 1 gene antibody (Cux1, transcription factor) for differentiated neurons in the upper layer. Scale bars = 50 μm. Right, quantification of the average line profiles throughout the cortex (n = 3). Normalization was performed taking into account the slice thickness. Displayed is the average plot profile.