Supplementary Figure 4 : Migration in vitro of the Fmr1−/− neurons is not affected.

From: FMRP regulates multipolar to bipolar transition affecting neuronal migration and cortical circuitry

Supplementary Figure 4

WT and Fmr1−/− cells were isolated from E14.5 embryonic cortices, left to form aggregates and embedded in Matrigel 18 h later. Sequential images show four time points of WT and Fmr1−/− neurons migrating away from the in vitro explants. Both average velocity and directionality (total path length from time 0 to time x/ net distance time 0 to time x) were not different between WT and Fmr1−/−­­ neurons: Average velocity: WT = 0.1537 μm/min ± 0.0226, n = 6 (71 neurons); Fmr1−/− = 0.1609 μm/min ± 0.01687, n = 5 (59 neurons); unpaired two tailed t-test: P = 0.8122, t = 0.2447, df = 9, n = number of explants. Directionality: WT 0.6629 ± 0.03070, arbitrary units; Fmr1−/− 0.6642 ± 0.06615 arbitrary units, P = 0.9857, t=0.01844, df=9. Scale bar = 10 μm