Supplementary Figure 2 : Absence of Fmrp does not affect proliferation of precursor cells at E14.5.

From: FMRP regulates multipolar to bipolar transition affecting neuronal migration and cortical circuitry

Supplementary Figure 2

a) Left, confocal images from WT and Fmr1−/− E14.5 mouse cortices stained with markers for the intermediate (Tbr2-positive) and radial (Sox2-positive) progenitor populations (anti-Tbr2, green; anti-Sox2, red). Right, the graph represents the ratio of Tbr2-positive to Sox2-positive in WT and Fmr1−/− cortices. Scale bars = 20 μm. (Values are mean ± SEM; two tailed unpaired t-test: P = 0.1503, t=1.776, df = 4, n = 3 WT animals and n = 3 Fmr1−/− animals). b) Histogram represents the percentage of cells transfected at E14.5 with pCAG-EGFP that are Ki67-positive in the VZ/SVZ/IZ of WT and Fmr1−/− cortices. (Values are mean ± SEM; two tailed unpaired t-test: P = 0.423, t = 0.86, df = 6, n = 3 WT animals and n = 3 Fmr1−/− animals).