Supplementary Figure 3 : AnkG-deficient nodes of Ranvier have Na+ channels that co-localize with AnkR and βI spectrin.

From: A hierarchy of ankyrin-spectrin complexes clusters sodium channels at nodes of Ranvier

Supplementary Figure 3

(a,b) Ank3F/F and Avil-Cre; Ank3F/F dorsal roots (a), and Ank3F/F and Six3-Cre; Ank3F/F optic nerve (b) immunostained using antibodies against Na+ channels (red), neurofascin (NF)/Caspr (blue), and AnkR (green). Nodes of AnkG-deficient axons have high densities of Na+ channels colocalized with AnkR. (c,d) Ank3F/F and Avil-Cre; Ank3F/F dorsal roots (c), and Ank3F/F and Six3-Cre; Ank3F/F optic nerve (d) immunostained using antibodies against Na+ channels (red), Caspr (blue), and βI spectrin (green). Some nodes (arrows) of AnkG-deficient axons have Na+ channels colocalized with βI spectrin. Scale bars, 10 µm.