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A common origin of synaptic vesicles undergoing evoked and spontaneous fusion

Nature Neuroscience volume 13, pages 1451–1453 (2010)Cite this article

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Abstract

There is a longstanding controversy on the identity of synaptic vesicles undergoing spontaneous versus evoked release. A recent study, introducing a new genetic probe, suggested that spontaneous release is driven by a resting pool of synaptic vesicles refractory to stimulation. We found that cross-depletion of spontaneously or actively recycling synaptic vesicle pools occurred on stimulation in rat hippocampal neurons and identified the recycling pool as a major source of spontaneous release.

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Figure 1: Synaptic vesicles labeled by spontaneous or activity-dependent uptake exhibit identical release kinetics on stimulation.
Figure 2: Synaptic vesicles endocytosed spontaneously and on stimulation recycle equally to the recycling (Rc) and resting (Rs) synaptic vesicle pools.
Figure 3: Synaptic vesicles undergoing spontaneous and activity-dependent recycling originate from the recycling pool.

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References

  1. Katz, B. & Miledi, R. J. Physiol. (Lond.) 203, 689–706 (1969).

    Article  CAS  Google Scholar 

  2. Murthy, V.N. & Stevens, C.F. Nat. Neurosci. 2, 503–507 (1999).

    Article  CAS  Google Scholar 

  3. Lou, X., Scheuss, V. & Schneggenburger, R. Nature 435, 497–501 (2005).

    Article  CAS  Google Scholar 

  4. Prange, O. & Murphy, T.H. J. Neurosci. 19, 6427–6438 (1999).

    Article  CAS  Google Scholar 

  5. Sara, Y., Virmani, T., Deak, F., Liu, X. & Kavalali, E.T. Neuron 45, 563–573 (2005).

    Article  CAS  Google Scholar 

  6. Groemer, T.W. & Klingauf, J. Nat. Neurosci. 10, 145–147 (2007).

    Article  CAS  Google Scholar 

  7. Fredj, N.B. & Burrone, J. Nat. Neurosci. 12, 751–758 (2009).

    Article  Google Scholar 

  8. Angleson, J.K. & Betz, W.J. J. Neurophysiol. 85, 287–294 (2001).

    Article  CAS  Google Scholar 

  9. Adie, E.J. et al. Assay Drug Dev. Technol. 1, 251–259 (2003).

    Article  CAS  Google Scholar 

  10. Howarth, M. & Ting, A.Y. Nat. Protoc. 3, 534–545 (2008).

    Article  CAS  Google Scholar 

  11. Martens, H. et al. J. Neurosci. 28, 13125–13131 (2008).

    Article  CAS  Google Scholar 

  12. Sankaranarayanan, S. & Ryan, T.A. Nat. Neurosci. 4, 129–136 (2001).

    Article  CAS  Google Scholar 

  13. Miesenböck, G., De Angelis, D.A. & Rothman, J.E. Nature 394, 192–195 (1998).

    Article  Google Scholar 

  14. Kim, S.H. & Ryan, T.A. Neuron 67, 797–809 (2010).

    Article  CAS  Google Scholar 

  15. Chung, C., Barylko, B., Leitz, J., Liu, X. & Kavalali, E.T. J. Neurosci. 30, 1363–1376 (2010).

    Article  CAS  Google Scholar 

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Acknowledgements

We thank K. Hardes for culturing hippocampal neurons, H. Martens (Synaptic Systems) for providing antibodies and E. Neher for critical reading of the manuscript. This work was supported by the Deutsche Forschungsgemeinschaft (Kl 1334/1-1 to J.K.). Y.H. is supported by a stipend from the Max Planck foundation and R.S. by a stipend from the International Max Planck Research School in Neurosciences Göttingen.

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Author notes

  1. Yunfeng Hua and Raunak Sinha: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany

    Yunfeng Hua, Raunak Sinha & Jürgen Klingauf

  2. Department of Cellular Biophysics, Institute for Medical Physics and Biophysics, University of Muenster, Muenster, Germany

    Yunfeng Hua, Raunak Sinha, Magalie Martineau, Martin Kahms & Jürgen Klingauf

Authors
  1. Yunfeng Hua
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  2. Raunak Sinha
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  3. Magalie Martineau
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  4. Martin Kahms
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  5. Jürgen Klingauf
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Contributions

Y.H. and R.S. designed, performed and analyzed the CypHer antibody and spH experiments. M.M. and M.K. designed, performed and analyzed the streptavidin-cypHer experiments. J.K. initialized the project. All of the authors wrote the paper.

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Correspondence to Jürgen Klingauf.

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The authors declare no competing financial interests.

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Supplementary Figures 1–5, Supplementary Notes 1–3, Supplementary Discussion and Supplementary Methods (PDF 496 kb)

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Hua, Y., Sinha, R., Martineau, M. et al. A common origin of synaptic vesicles undergoing evoked and spontaneous fusion. Nat Neurosci 13, 1451–1453 (2010). https://doi.org/10.1038/nn.2695

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