Abstract
Can dendrites grow in mature cortex? We used chronic in vivo imaging to follow pyramidal neurons before and after cortical deletion of the Pten tumor suppressor gene in mature mice. We found that Pten/mTOR signaling uniquely regulates the growth of layer 2/3 apical dendrites; no effects of gene deletion were observed on basal dendrites of these pyramidal neurons or along layer 5 apical dendrites.
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Acknowledgements
We thank E.M. Callaway and K. Bochmann for providing the ZnG reporter mice. This work was supported by the US National Eye Institute (EY016052), the Esther and Joseph Klingenstein Foundation, the University of California Los Angeles Center for Autism Research and Treatment, the US National Institute for Mental Health, the National Institute of Child Health and Human Development, the National Institute on Deafness and Other Communication Disorders, the National Institute of Neurological Disorders and Stroke (MH68172, University of California Los Angeles Studies to Advance Autism Research and Treatment Center) and a Ruth L. Kirchstein National Research Service Award (GM07185).
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D.K.C., M.G. and J.T.T. designed the experiments. D.K.C. and J.T.T. carried out all of the imaging experiments, analyzed the data and wrote the manuscript. M.P. performed the pS6 immunostaining. M.M. and S.T.C. carried out the laser-capture quantitative real-time PCR. M.P. and X.L. provided the mice.
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Chow, D., Groszer, M., Pribadi, M. et al. Laminar and compartmental regulation of dendritic growth in mature cortex. Nat Neurosci 12, 116–118 (2009). https://doi.org/10.1038/nn.2255
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DOI: https://doi.org/10.1038/nn.2255
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