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Neuronal release of proBDNF

Nature Neuroscience volume 12, pages 113–115 (2009)Cite this article

Abstract

Pro–brain-derived neurotrophic factor (proBDNF) and mature BDNF utilize distinct receptors to mediate divergent neuronal actions. Using new tools to quantitate endogenous BDNF isoforms, we found that mouse neurons secrete both proBDNF and mature BDNF. The highest levels of proBDNF and p75 were observed perinatally and declined, but were still detectable, in adulthood. Thus, BDNF actions are developmentally regulated by secretion of proBDNF or mature BDNF and by local expression of p75 and TrkB.

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Figure 1: Detection of proBDNF and mature BDNF in mouse hippocampus and secretion of proBDNF and mature BDNF by cultured hippocampal neurons.
Figure 2: Developmental regulation of proBDNF and p75 expression.

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Acknowledgements

We thank K. Teng and members of the Hempstead laboratory for their input. These studies were supported by grants from the National Institute of Neurological Disorders and Stroke (NS30687 to B.L.H. and NS52819 to F.S.L.), National 973 Basic Research Program of China (No. 2006CB503803 and 2009CB941403 to Z.-Y.C.) and the Intramural Program of the National Institute of Child Health and Human Development and the National Institute of Mental Health (to B.L.).

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Affiliations

  1. Department of Medicine, Weill Cornell Medical College, 1300 York Ave., New York, 10065, New York, USA

    Jianmin Yang, Chia-Jen Siao, Tina Marinic, Kelly McGrath & Barbara L Hempstead

  2. Department of Psychiatry, Weill Cornell Medical College, 1300 York Ave., New York, 10065, New York, USA

    Deqiang Jing, Zhe-Yu Chen & Francis S Lee

  3. Developmental Biology, Memorial Sloan Kettering Cancer Center, 1275 York Ave., New York, 10065, New York, USA

    Willie Mark

  4. National Cancer Institute Frederick, Building 560, Frederick, 21702, Maryland, USA

    Lino Tessarollo

  5. National Institute of Child Health and Human Development, MSC 3714, Bethesda, 20892-4480, Maryland, USA

    Guhan Nagappan & Bai Lu

Authors
  1. Jianmin Yang
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  2. Chia-Jen Siao
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  3. Guhan Nagappan
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  4. Tina Marinic
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  5. Deqiang Jing
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  6. Kelly McGrath
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  7. Zhe-Yu Chen
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  8. Willie Mark
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  9. Lino Tessarollo
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  10. Francis S Lee
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  11. Bai Lu
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  12. Barbara L Hempstead
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Contributions

C.-J.S., T.M., K.M., Z.-Y.C., W.M. and L.T. generated the Bdnf-HA mouse; G.N., B.L. and J.Y. generated and characterized the pro-domain antibody; J.Y., G.N. and D.J. performed the experiments; and B.L.H., B.L., F.S.L. and J.Y. designed the experiments and wrote the manuscript.

Corresponding author

Correspondence to Barbara L Hempstead.

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Supplementary Text and Figures

Supplementary Figures 1–3 and Supplementary Methods (PDF 4534 kb)

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Yang, J., Siao, CJ., Nagappan, G. et al. Neuronal release of proBDNF. Nat Neurosci 12, 113–115 (2009). https://doi.org/10.1038/nn.2244

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