Spike timing–dependent long-term depression requires presynaptic NMDA receptors

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Abstract

NMDA receptors are necessary for both synaptic potentiation and depression, but the precise location of these receptors has not been established. By loading MK-801 into pre- or postsynaptic neurons during paired recordings of synaptically connected layer 4 and layer 2/3 neurons in mouse barrel cortex, we found that synaptic potentiation requires postsynaptic, but not presynaptic, NMDA receptors, whereas synaptic depression requires presynaptic, but not postsynaptic, NMDA receptors.

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Figure 1: NMDA receptor dependence of timing-dependent plasticity in barrel cortex.
Figure 2: t-LTP and t-LTD require postsynaptic and presynaptic NMDA receptors, respectively.

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Acknowledgements

This work was supported by the Medical Research Council (grant number G0400571) and by an equipment grant from the Alzheimer's Research Trust. A.R.-M. holds a Marie Curie Intra-European Fellowship.

Author information

A.R.-M. conducted the experiments and analyzed the data. A.R.-M. and O.P. designed the experiments and wrote the manuscript.

Correspondence to Antonio Rodríguez-Moreno or Ole Paulsen.

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Supplementary Figures 1–3 and Supplementary Methods (PDF 194 kb)

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Rodríguez-Moreno, A., Paulsen, O. Spike timing–dependent long-term depression requires presynaptic NMDA receptors. Nat Neurosci 11, 744–745 (2008) doi:10.1038/nn.2125

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