Antibiotic resistance is a major public health threat, further complicated by unexplained treatment failures caused by bacteria that appear antibiotic susceptible. We describe an Enterobacter cloacae isolate harbouring a minor subpopulation that is highly resistant to the last-line antibiotic colistin. This subpopulation was distinct from persisters, became predominant in colistin, returned to baseline after colistin removal and was dependent on the histidine kinase PhoQ. During murine infection, but in the absence of colistin, innate immune defences led to an increased frequency of the resistant subpopulation, leading to inefficacy of subsequent colistin therapy. An isolate with a lower-frequency colistin-resistant subpopulation similarly caused treatment failure but was misclassified as susceptible by current diagnostics once cultured outside the host. These data demonstrate the ability of low-frequency bacterial subpopulations to contribute to clinically relevant antibiotic resistance, elucidating an enigmatic cause of antibiotic treatment failure and highlighting the critical need for more sensitive diagnostics.
Antibiotic resistance threatens the delivery of safe and effective healthcare1 and is projected to lead to 10 million
annual deaths worldwide by 20502.
Failure of antibiotic treatment results in increased length of patient stay, healthcare
costs and mortality2. Multi-drug
resistant Enterobacter spp. have increasingly emerged as a cause of
Phenotypically resistant subpopulation
A strain of E. cloacae was isolated from a renal transplant
recipient10 and was
observed to harbour a distinct subpopulation with resistance to colistin,
visualized as numerous colonies within the zone of inhibition on testing by
colistin Etest (we refer to the strain as ‘R/S’, to indicate the
presence of both resistant and susceptible subpopulations) (Fig. 1a). This was not observed with either
colistin-susceptible or -resistant (Supplementary Fig. 1) clinical strains. A population
analysis profile (PAP) of R/S, in which a strain is assayed for survival on agar
plates with increasing amounts of antibiotics, revealed a major proportion of
the bacteria (>90%) susceptible to
1 µg ml–1 colistin and a highly
resistant subpopulation able to withstand at least
500 µg ml–1 colistin (Fig. 1b). This was in contrast to the susceptible
strain, which was uniformly killed by
1 µg ml–1 colistin, and the resistant
strain, which was uniformly killed by
200 µg ml–1 colistin. The proportion
of the R/S colistin-resistant subpopulation was increased to upwards of
80% on exposure to colistin (Fig.
1c). Further analysis revealed that this increase was due to an initial
selection against the colistin-susceptible population over the first 2 h
of antibiotic exposure, followed by robust replication and expansion of the
resistant population in the presence of the drug (Fig. 1d). Importantly, this suggests that the resistant cells are
not persisters, which do not significantly expand in number during antibiotic
To determine whether the increase in the proportion of the resistant subpopulation occurs during antibiotic treatment in vivo, we infected mice with R/S and treated them with colistin or PBS. In colistin-treated mice, we observed a significant increase in the frequency of the resistant subpopulation of bacteria isolated from the peritoneum (Fig. 1e) and liver (Supplementary Fig. 4). Surprisingly, there was also a robust increase in the resistant subpopulation during in vivo infection in the absence of colistin treatment (Fig. 1e and Supplementary Fig. 4). By 48 h, the percentage of the resistant subpopulation increased from <10% to >80% (Supplementary Fig. 5). These results highlight the process of infection as leading to a significant increase in the frequency of an antibiotic-resistant subpopulation of bacteria.
Resistance to innate immune defences
Various host pressures could be responsible for the increase in the colistin-resistant subpopulation during infection. As macrophages are a major component of the early immune response14, we tested their role by depleting these cells with clodronate liposomes15 (Supplementary Fig. 6) and subsequently infecting mice with R/S. In contrast to bacteria recovered from mice treated with control liposomes, which demonstrate a robust increase in the frequency of the resistant subpopulation, those recovered from macrophage-depleted mice showed no such increase (Fig. 2a). Based on these results, we next determined whether macrophages were sufficient to cause the increase in the resistant subpopulation, by infecting them in vitro. During macrophage infection, the colistin-resistant subpopulation increased to 40% within only 2 h (Fig. 2b). This rise was dependent on internalization of the bacteria, because preventing phagocytosis with cytochalasin D abrogated this phenomenon (Fig. 2b). Therefore, macrophages are both required and sufficient for the increased frequency of the resistant subpopulation during infection, underlining a role for a specific innate immune cell type in this process.
Macrophages possess many antimicrobials16, and we hypothesized that specific components would be required for the increase in the frequency of the resistant subpopulation, testing reactive oxygen species (formed after treatment with hydrogen peroxide), lysozyme and the murine cationic antimicrobial peptide CRAMP. All of these antimicrobials resulted in a dose-dependent increase in the frequency of the colistin-resistant subpopulation in vitro (Fig. 2c–e), as did LL-37, the human orthologue of CRAMP (Supplementary Fig. 7). These results led us to test whether the antimicrobials were responsible for the increase in the resistant subpopulation during in vivo infection. We infected wild-type (WT) and triple knockout (TKO) mice lacking a functional NADPH oxidase (which leads to the production of reactive oxygen species17), lysozyme and CRAMP. TKO mice were more susceptible to infection by R/S as they harboured over ten times more bacteria than WT mice (Supplementary Fig. 8), demonstrating the importance of these antimicrobials in host defence. Although a robust increase in the frequency of the resistant subpopulation was observed in wild-type mice, this was abrogated in TKO mice (Fig. 2f). The frequency of the resistant subpopulation in mice lacking one of these three antimicrobials was not significantly different from that in WT mice, but it was decreased in double KO mice lacking the NADPH oxidase and CRAMP or lysozyme (Supplementary Fig. 9). These data identify a role for specific host innate immune antimicrobials in the increase of an antibiotic-resistant subpopulation during in vivo infection.
Subpopulation-mediated antibiotic failure
To determine the relevance of the increase in frequency of the resistant subpopulation during in vivo infection, we tested whether the R/S strain was able to resist colistin treatment. We infected mice with either R/S or a colistin-susceptible strain, and treated the mice with PBS (as a control) or high doses of colistin after establishment of infection to simulate the progression of infection and treatment in the clinic. The levels of the susceptible strain in the peritoneum (Fig. 3a) and liver (Fig. 3b) were significantly reduced by colistin treatment. In contrast, the R/S strain was refractory to treatment with colistin as its levels were unchanged between the treated and untreated groups (Fig. 3a,b). In a timecourse experiment, the level of the susceptible strain was reduced by 3 logs at 42 h, whereas the level of the R/S strain was not diminished by colistin treatment, but instead increased by roughly tenfold (Supplementary Fig. 10). These data suggest that the presence of the resistant subpopulation results in inefficacy of colistin to reduce bacterial levels in vivo. Furthermore, these results provide in vivo evidence that the resistant subpopulation does not behave like persisters, which do not significantly expand in number during antibiotic treatment.
We next tested whether the role of the host immune system in the increase of the resistant subpopulation was directly responsible for the inefficacy of antibiotic therapy. We first found that colistin treatment of R/S-infected mice could cause a significant reduction in bacterial levels if initiated at the time of infection (before the increase in the frequency of the resistant subpopulation), but not if it was delayed until only 4 h after infection (Fig. 3c). However, in macrophage-depleted mice, treatment with colistin at 4 h became effective, leading to a reduction in bacterial levels (Fig. 3c) and indicating that the host-driven increase in the frequency of the resistant subpopulation is responsible for the inefficacy of antibiotic treatment.
To further test the relevance of this phenomenon in vivo, we infected mice with a lethal dose of bacteria and treated with either PBS or colistin after 12 h. Both the susceptible and R/S strains led to lethal infections in the absence of colistin (Fig. 3d,e). In the presence of colistin, only mice infected with the susceptible strain were rescued (Fig. 3d), whereas those infected with R/S still succumbed to infection within 100 h (Fig. 3e). These data demonstrate the impact of an antibiotic-resistant subpopulation in mediating a lethal infection during high-dose antibiotic treatment.
PhoQ-dependent resistant subpopulation
We next set out to determine the molecular mechanism underlying the phenotype of
the resistant subpopulation. RNAseq analysis was conducted (Supplementary Fig. 3 and
1) to determine whether there were transcriptional differences
between the susceptible and resistant subpopulations of R/S. In total, this
analysis revealed 325 genes upregulated and 360 genes downregulated in the
resistant subpopulation as compared to the susceptible subpopulation (Supplementary Table 2).
This approach should detect differences between the two subpopulations, but it
may also identify expression differences due to colistin treatment. Among the
upregulated genes, we noticed a signature (Supplementary Table 3)
associated with the two-component histidine kinase PhoQ (refs 18,
To interrogate the potential contribution of PhoQ to the R/S resistance phenotype, we examined the colistin resistance profile of ΔphoQ. Strikingly, the ΔphoQ strain exhibited a complete absence of the resistant subpopulation by Etest, while the susceptible subpopulation remained unaffected, as the border of the zone of clearing was unaltered from that of WT R/S (Fig. 4a). Complementation with phoQ restored the presence of the resistant subpopulation (Fig. 4a). This was also confirmed by PAP, where ΔphoQ lacked the resistant subpopulation present in R/S and behaved in a similar manner to the susceptible strain (Fig. 4b). Importantly, R/S and ΔphoQ harboured equivalent levels of persisters, clearly indicating that the colistin-resistant subpopulation (which depends on PhoQ) is not made up of persister cells (Supplementary Fig. 13). The phoQ mutant also exhibited no colistin-resistant subpopulation after exposure to host antimicrobials (Supplementary Fig. 14a), during macrophage infection (Supplementary Fig. 14b) or during in vivo infection (Supplementary Fig. 14c). Without the presence of the resistant subpopulation, ΔphoQ was susceptible to colistin treatment in vivo, exhibiting a significantly decreased bacterial load (Fig. 4c). Furthermore, the ability of colistin to rescue mice from an otherwise lethal inoculum was restored during infection with ΔphoQ (Fig. 4d). Thus, the presence of the colistin-resistant subpopulation is dependent on PhoQ, which is required for a lethal drug-resistant infection.
Undetected resistant subpopulation
The size of the resistant subpopulation can vary greatly between strains, as exemplified by a distinct E. cloacae clinical isolate (termed R/S-lo) that harbours a colistin-resistant subpopulation comprising between 0.01 and 0.001% of the total population (Fig. 5a), over 1,000-fold less prevalent than that of R/S when grown in media without antibiotic. Similar to R/S, the increase of the R/S-lo resistant subpopulation in the presence of colistin (Supplementary Fig. 15) was due to initial selection against the susceptible subpopulation followed by expansion of the resistant subpopulation (Supplementary Fig. 16). The frequency of the resistant subpopulation was similarly increased by treatment with H2O2, lysozyme, CRAMP and LL-37 (Supplementary Fig. 17), during macrophage infection (Supplementary Fig. 18) and during in vivo infection of mice (Supplementary Fig. 19), and was greatly diminished in macrophage-depleted (Supplementary Fig. 20) and TKO mice (Supplementary Fig. 21). These data revealed that, similar to R/S, the frequency of the resistant subpopulation of R/S-lo is increased by colistin as well as the activity of specific host innate immune components. During in vivo infection, although the levels of a susceptible strain were significantly reduced by colistin treatment, the levels of R/S-lo were unaffected (Supplementary Fig. 22). These data directly correlated with a failure of colistin therapy to rescue R/S-lo infected mice from a lethal infection (Fig. 5c), whereas mice infected with a susceptible strain were completely rescued (Fig. 5b). Importantly, unlike R/S, R/S-lo was clinically classified as being susceptible to colistin, as the resistant subpopulation (present at a frequency of only 1 in 10,000 c.f.u.) was not detected by Etest (Fig. 5d). Therefore, this seemingly colistin-susceptible strain, harbouring an undetected resistant subpopulation, is capable of causing an antibiotic-resistant and lethal infection in vivo.
It is worrisome that R/S-lo was not identified as colistin-resistant, and we wondered whether the resistant population could be detected by diagnostic testing when it is more frequent during host infection. We directly plated peritoneal lavage samples from infected mice in the absence of subculture and were able to detect the R/S-lo resistant subpopulation by Etest, as indicated by colonies within the zone of inhibition (Fig. 5e,g). In contrast, when these samples were processed by the clinical microbiology laboratory (as would occur with a sample from a human patient and including a critical subculture step), Etest could no longer detect the diminished resistant subpopulation (Fig. 5f,g). Strikingly, these data reveal how and when detection of the resistant subpopulation can be missed during routine diagnostic testing and how this can translate into an unexplained failure of antibiotic therapy.
The findings presented here highlight the role of a minor colistin-resistant bacterial subpopulation in mediating antibiotic treatment failure in vivo. This resistant subpopulation is genetically identical to the susceptible subpopulation, but exhibits differences in gene expression and lipid A modification. Furthermore, the presence of this resistant subpopulation is dependent on the histidine kinase PhoQ. The data also highlight an unexpected role for specific host innate immune components (lysozyme, CRAMP and H2O2) in the increase of the antibiotic-resistant subpopulation during infection. The increase in the frequency of the resistant subpopulation induced by host immune pressure in vivo was shown to be critical for eventual failure of colistin therapy.
Like bacterial persistence, the phenotypic resistance phenomenon we describe involves a resistant subpopulation, but there are important differences. Persistence involves a small subpopulation of bacteria that are tolerant to a drug due to a state of low metabolic activity, with no or limited replication28. Wakamoto et al. showed that, in some cases, persisters can replicate, although it is at a very low rate and is insufficient to cause an overall increase in the numbers of the population11. In contrast, we describe a resistant subpopulation that rapidly replicates both in vitro and in vivo in the presence of antibiotic and leads to a very significant overall increase in bacterial population level (Fig. 1d and Supplementary Fig. 10). Furthermore, we directly show that the PhoQ-dependent colistin-resistant subpopulation is distinct from persisters, which are also present but independent of PhoQ (Supplementary Fig. 13). Several papers have recently demonstrated the importance of persisters as a reservoir of infection during antibiotic treatment in vivo12, which can continue to replicate after treatment has been stopped, leading to relapse13. In contrast, we demonstrate that the colistin-resistant subpopulation described here facilitates bacterial growth and subsequent host lethality, even in the presence of antibiotic. Persistence has also been linked to immune pressure, as bacterial populations within macrophages can have increased numbers of persisters29. We observe a similar link; both in vitro and in vivo, specific host antimicrobials lead to an increased frequency of the resistant subpopulation. Taken together, both persisters and the resistant subpopulation described here highlight the ability of a minority of a bacterial population to exert a striking effect on the outcome of infection and antibiotic treatment. Persisters are kept at bay by antibiotic treatment and form a reservoir that can cause relapse, but the colistin-resistant subpopulation described here has the ability to cause acute infection and lethality during the course of antibiotic treatment.
We propose to refer to the resistance phenomenon described here as ‘clonal
heteroresistance’. The phenomenon of heteroresistance, in which a resistant
subpopulation exhibits an increased level of antibiotic resistance relative to the
larger susceptible subpopulation, was described as far back as 1947 (ref. 30). However, its relevance to infection and
resistance has remained unclear, and even its definition has been debated. We use
the term clonal heteroresistance to distinguish the phenomenon we describe from the
blanket term heteroresistance, which is often used to refer to mixed populations of
genetically distinct bacteria31,
E. cloacae strain R/S was isolated from a blood sample from a renal transplant recipient at Emory University Hospital, Atlanta, GA. E. cloacae R/S-lo, the colistin-susceptible strain Mu819 and the colistin-resistant strain Mu117 were isolated from urine samples from patients at other Atlanta hospitals.
All bacterial strains were streaked on Mueller–Hinton (MH) agar plates and grown in MH medium at 37 °C in a shaking incubator from a single colony before each experiment. Numbers of c.f.u. were determined by plating dilutions on MH agar plates incubated at 37 °C and then counting bacterial colonies at the lowest distinguishable dilution.
To generate strain ΔphoQ, 600–700 bp upstream and downstream fragments of the genomic region surrounding phoQ were PCR-amplified with primers 81 and 118, and 82 and 119, respectively (Supplementary Table 4), and fused with the hygromycin resistance cassette HmR amplified from vector pMQ310 with primers 79 and 80 (ref. 35) using splicing by overlap extension (SOE) PCR (ref. 36). The suicide vector pEXR6K was generated by replacing the pMB1 ori from PCR linearized plasmid pEX100T (ref. 37) using primers 110 and 111 with the R6K ori amplified from plasmid pMQ310 with primers 108 and 109 using the Gibson Assembly Cloning Kit (Invitrogen). The HmR construct was inserted into SmaI (New England Biolabs) digested pEXR6K by Gibson assembly and the resulting plasmid was transformed to strain R/S by electroporation. Transformants were selected on MH agar containing 150 µg ml–1 hygromycin (Sigma) then passaged to Luria-Bertani (LB) agar containing 20% sucrose and no NaCl to counterselect for vector loss. Chromosomal replacement of phoQ with the hygromycin marker was confirmed by Sanger sequencing. To generate strain phoQ-comp, the promoter region of the phoPQ operon was amplified with primers 142 and 143 and fused by SOE PCR to gene phoQ amplified with primers 144 and 145. The resulting construct was inserted to plasmid pBAV-1K-T5-GFP (ref. 38) PCR linearized with primers 146 and 147 to create the complementation vector. The vector was transformed to strain ΔphoQ by electroporation and selected on MH agar containing 90 µg ml–1 kanamycin (Sigma).
Antibiotic susceptibility testing
The colistin susceptibility of all strains was determined using the Etest method. Briefly, the inoculum was prepared from colonies grown on a 5% sheep blood agar plate (Remel, Lenexa, KS) for 18 h. Several colonies were suspended in 0.9% sterile saline (Remel) and adjusted to a concentration equivalent to a 0.5 McFarland turbidity standard. The suspension was used to streak a 100-mm-diameter MH agar plate and the Etest strip (bioMérieux) was placed on it. The plate was incubated at 35 °C for 20 h and the minimum inhibitory concentration (MIC) was read where inhibition of growth intersected the Etest strip. Small colonies that grew within the zone of inhibition were included in the MIC determination. Etest analyses of samples from mouse infections were plated directly from peritoneal lavage samples without subculturing. PAPs were performed by growing bacteria to mid-log phase and then plating on MH agar containing various concentrations of colistin. Percentage colistin resistance was calculated as the number of bacteria that grew on 100 µg ml–1 colistin divided by the number of bacteria that grew on MH alone.
WT C57BL/6J mice were purchased from Jackson Laboratories and used at age 8–10 weeks; all experiments used age- and sex-matched mice. TKO mice deficient in the gp91 component of the NADPH oxidase, lysozyme and CRAMP, as well as double knockout mice lacking two of the indicated antimicrobials, were derived by crossing cybb−/− (gp91, Jackson Laboratories), lysM−/− (lysozyme; generously provided by D. Portnoy, UC Berkeley) and cnlp−/− (CRAMP; Jackson Laboratories) mice. TKO mice were investigated for health defects by histology and bacterial culture of various organs, with no overt health differences observed in uninfected TKO mice when compared to WT. Mice were housed under specific-pathogen-free conditions in filter-top cages at Yerkes National Primate Center, Emory University, and provided food and water ad libitum. All experimental procedures were approved by the Emory University Institutional Animal Care and Use Committee (IACUC). Sample size, as reported in the figure legends, was determined by allowing for significance by the Mann–Whitney test (n ≥ 4) while minimizing the number of animals used, and five mice were thus used per group for the majority of experiments. No randomization or blinding was done in the animal studies.
Approximately 5 × 107 c.f.u. were administered per mouse for infections to quantify the bacterial load, and approximately 2 × 108 c.f.u. were administered for survival experiments. Bacterial inocula were suspended in PBS and 100 μl was inoculated intraperitoneally (i.p.) to each mouse. Colistin methanesulfonate was injected i.p. in 100 μl PBS at a dosage of 10 mg per kg per dose. Mice were monitored by weight and were euthanized if found to be below 80% starting weight, as mandated by the IACUC protocol. Mice were euthanized, and the liver, spleen and peritoneal lavage samples were collected into sterile PBS. Solid organ samples were homogenized using a tissue-tearor (BioSpec), and all samples were plated for c.f.u. and per cent colistin resistance.
Macrophages were depleted from mice using clodronate liposomes (clodronateliposomes.com). Mice were injected with 200 µl liposomes i.p. 3 days before infection and then injected again with 100 µl liposomes i.v. 1 day before infection. Mice were infected i.p. for 8 h before peritoneal lavage fluid was collected and plated for c.f.u. Part of this sample was also used for flow cytometry to confirm macrophage depletion.
Macrophages were derived from the bone marrow of mice. Briefly, femurs from mice were removed and whole bone marrow was flushed out. The bone marrow cells were grown in media containing DMEM, 10% fetal bovine serum (FBS) and macrophage colony-stimulating factor (M-CSF), which induces the differentiation and growth of macrophages. After confluent layers of macrophages were derived, cells were plated into 24-well plates at 3 × 105 cells per well. Bacteria were added to the wells at 3 × 106 c.f.u. per well for a multiplicity of infection (MOI) of 10:1. Plates were centrifuged to synchronize the infection. After 30 min, the macrophages were washed and 100 µg ml–1 of gentamicin was added to the media to remove and prevent the growth of extracellular bacteria. At 1, 2 and 4 h post-infection, macrophages were incubated with 1% saponin in PBS for 2 min to lyse open cells and remove bacteria. Samples were then plated for c.f.u., and per cent colistin resistance calculated. To prevent internalization of bacteria, some wells were pretreated with 1 µg ml–1 cytochalasin D for 30 min before the addition of bacteria.
Peritoneal lavage fluid was stained with F4/80-PE/Cy7 (BM8) (Biolegend) and CD11b-APC/A700 (M1I70) (eBioscience) antibodies for 35 min. Red blood cells (RBCs) were lysed with RBC lysis buffer (Becton Dickinson) for 5 min. Cells were fixed with 1% paraformaldehyde and analysed on an LSRII flow cytometer (BD). Macrophages were defined as F4/80+CD11b+ cells.
DNA and RNA isolation
An overnight liquid culture of R/S grown at 37 °C in MH broth was back-diluted in triplicate to either fresh MH broth or MH broth containing 100 µg ml–1 colistin to enrich for susceptible or colistin-resistant bacteria, respectively. Cultures were grown to exponential phase at 37 °C and collected for DNA and RNA isolation. Numbers of c.f.u.were calculated as above. DNA was isolated using the DNEasy Blood and Tissue Kit (Qiagen) following the Gram negative bacteria protocol with RNase treatment. RNA was isolated using a modified phase extraction method39 with initial incubation in TriReagent (Zymo) followed by phase separation with chloroform. RNA was precipitated from the aqueous phase with isopropanol and 1.2 M NaCl at 4 °C, and further purified with the Directzol RNA Kit (Zymo) following the recommended DNase treatment step.
DNA and RNA sequencing
Sample integrities were verified with the Agilent 2100 Bioanalyzer. DNA libraries were prepared using the NexteraXT DNA kit (Illumina). For RNA libraries, samples were first depleted of ribosomal RNAs using the Ribo-Zero rRNA Removal Kit (Illumina) and libraries prepared using the EpiCentre ScriptSeq Complete (Bacteria) Low Input kit (Illumina). Next-generation short sequence reads were generated with the Illumina HiSeq 1000 platform at the Yerkes National Primate Research Center Nonhuman Primate Genomics Core (http://www.yerkes.emory.edu/nhp_genomics_core/). Long sequence reads were generated with the PacBio II platform using P5-C3 chemistry at the Duke University Sequencing and Genomic Technologies Shared Resource.
De novo genome assembly and sequence analysis
A hybrid de novo assembly was performed using both Illumina and PacBio data using Celera Assembler version 8.2 (ref. 40). The sequence data resolved into two contigs, one representing the chromosome and the other representing the plasmid. The quality of the assembly was confirmed by analysis using the ALE tool41. The assembly was automatically annotated using the NCBI prokaryotic annotation pipeline. Illumina whole shotgun sequences of the samples enriched for colistin resistance (COL) and colistin susceptibility (MH) were aligned against the assembled genome using bwa-0.7.12 (ref. 42) and visualized with the samtools-1.2 mpileup function43. Single nucleotide polymorphisms between the assembled genome and short sequence reads were manually analysed to determine sequence conservation between COL and MH samples.
Single-end Illumina libraries from reverse-transcribed RNA were mapped against the Enterobacter de novo assembled reference using Bowtie2 (ref. 44). Differential gene expression between the three colistin-treated strains and controls was quantified by the cufflinks/cuffdiff tools in CufflinksVersion 2.2.1 (refs 45,46). Sequences of differentially expressed genes with significant q-values were analysed with Blast2Go software version 3.1.3 to identify the Escherichia coli gene orthologue and putative function47.
Quantitative reverse transcriptase PCR (qRT-PCR)
RNA was collected as described above. One-step qRT-PCR was performed using the Power SYBR Green RNA-to-Ct kit (Applied Biosystems) with primers (Supplementary Table 4) on a StepOnePlus Real-time PCR System (Applied Biosystems) according to the manufacturer's instructions. rpoD was used as the internal control gene48. Relative expression was calculated as 2−(ΔCt) (ref. 49).
Isolation and analysis of 32P lipid A species
E. cloacae strains were grown overnight in MH broth, diluted 1:400 in fresh MH broth containing appropriate selective antibiotics. For induction of the resistant phenotype, 100 µg ml–1 colistin (Sigma) was used. E. coli W3110 and WD101 strains were grown in LB broth overnight followed by a 1:100 dilution in fresh LB medium. After dilutions, cells were immediately labelled with 2.5 µCi ml–1 of inorganic 32P-phosphate (Perkin Elmer) and collected at absorbance at 600 nm (A600) = 0.5 (E. cloacae) or A600 = 1.0 (E. coli). Lipid A extraction, separation and visualization was performed as described previously50. Briefly, lipid A extraction was carried out by mild acidic hydrolysis and spotted onto a silica thin-layer chromatography plate (10,000 c.p.m. per lane). Labelled lipid A species were separated using a solvent mixture of chloroform, pyridine, 88% formic acid and water (50:50:16:5). The thin-layer chromatography plate was exposed to a phosphoimager screen and visualized by phosphoimaging analysis (Bio-Rad PMI). The analysed images were cropped to aid in data analysis (for the full unaltered images see Supplementary Fig. 24).
Statistical analyses were performed using Prism 5 (Graphpad Software). The significance of the mouse experiments was determined with the Mann–Whitney test, as not all data were normally distributed, and all in vitro experiments were analysed using the two-tailed student's t-test (for data with a normal distribution). All experiments were repeated at least two to three times (and up to ten times). All replicates shown are biological replicates.
The authors thank S. Satola, M. Farley and the Georgia Emerging Infections Program for providing Enterobacter cloacae strains Mu117, Mu819 and R/S-lo, P. Rather for providing plasmid pMQ310, the Yerkes Nonhuman Primate Genomics Core for help with DNA sequencing and analysis, and C.-Y. Chin and D. Bonenberger for breeding and genotyping knockout mice. The authors also thank R. Ahmed, A. Grakoui and W. Shafer for comments and revisions of the manuscript. D.S.W. is supported by a Burroughs Wellcome Fund Investigator in the Pathogenesis of Infectious Disease award, VA Merit Award I01 BX002788 and National Institutes of Health (NIH) grant AI098800. E.K.C. is supported by the National Institute of Allergy and Infectious Diseases of the NIH under award no. T32AI106699. M.S.T. is supported by the NIH (grants nos RO1AI064184, RO1AI76322 and R21AI11987) and the Army Research Office (grant no. 61789-MA-MUR). T.D.R. and K.V. were supported by 1U01CI000906-05 ‘Emerging Infections Program (EIP) PPACA: Enhancing Epidemiology and Laboratory Capacity’ funding from the Emory Public Health Bioinformatics Fellowship. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH, the Department of Veterans Affairs or the Centers for Disease Control and Prevention.
Supplementary Tables 1–4