Bacteria growing under different conditions experience a broad range of demand on the rate of protein synthesis, which profoundly affects cellular resource allocation. During fast growth, protein synthesis has long been known to be modulated by adjusting the ribosome content, with the vast majority of ribosomes engaged at a near-maximal rate of elongation. Here, we systematically characterize protein synthesis by Escherichia coli, focusing on slow-growth conditions. We establish that the translational elongation rate decreases as growth slows, exhibiting a Michaelis–Menten dependence on the abundance of the cellular translational apparatus. However, an appreciable elongation rate is maintained even towards zero growth, including the stationary phase. This maintenance, critical for timely protein synthesis in harsh environments, is accompanied by a drastic reduction in the fraction of active ribosomes. Interestingly, well-known antibiotics such as chloramphenicol also cause a substantial reduction in the pool of active ribosomes, instead of slowing down translational elongation as commonly thought.
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The authors are grateful for discussions with numerous colleagues including Z. Ignatova, S. Klumpp, S. Pedersen, S. Schink, J. Silverman, M. Scott, R. Young and members of the Hwa laboratory at various stages of this work. This research is supported by NIH grant R01GM109069 (to T.H.) and grant 31530081 of the National Natural Science Fund of the People's Republic of China (NSFC; to Y.P.W.). K.F. acknowledges support from NIH grant R01GM072528. J.R.W. acknowledges NIH grant GM118850. M.Z. acknowledges financial support from the China Scholarship Council (CSC; 201306010039).
The authors declare no competing financial interests.
Supplementary Notes 1–5, Supplementary Tables 1–12, Supplementary Figures 1–20, Supplementary References (PDF 4670 kb)
Proteome abundances of individual r-proteins under nutrient limitation and Cm inhibition. (XLSX 48 kb)
Proteome abundance of individual r-proteins of the reference condition. (XLSX 64 kb)
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Dai, X., Zhu, M., Warren, M. et al. Reduction of translating ribosomes enables Escherichia coli to maintain elongation rates during slow growth. Nat Microbiol 2, 16231 (2017). https://doi.org/10.1038/nmicrobiol.2016.231
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