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Co-transfer of blaNDM-5 and mcr-1 by an IncX3–X4 hybrid plasmid in Escherichia coli

Nature Microbiology volume 1, Article number: 16176 (2016) Cite this article

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Abstract

Carbapenem and colistin are the last-resort antibiotics used for treating multidrug-resistant Gram-negative pathogens. Here, we report, for the first time, co-transfer of resistance to both classes of antibiotics by a mobile IncX3–X4 hybrid plasmid in an Escherichia coli isolate. Spread of such a plasmid is of great concern for clinical therapy, and heightened efforts are needed to control its dissemination.

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Figure 1: Genetic features of pCQ02-121 and proposed models for its formation.

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Acknowledgements

This work was supported by the National Natural Science Foundation and Natural Science Foundation of Guangdong Province, China (U1201214), the Program for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China (IRT13063), the Natural Science Foundation of Guangdong Province (S2012030006590) and the National Basic Research Program of China (2016YFC1200100).

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Author notes

  1. Jian Sun and Run-Shi Yang: †These authors contributed equally to this work.

Authors and Affiliations

  1. National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, 510642, Guangzhou, China

    Jian Sun, Run-Shi Yang, Liang-Xing Fang, Jing Xia, Liang Li, Xiao-Yue Lv, Jia-Hong Duan, Xiao-Ping Liao & Ya-Hong Liu

  2. College of Veterinary Medicine, South China Agricultural University, 510642, Guangzhou, China

    Jian Sun, Run-Shi Yang, Liang-Xing Fang, Jing Xia, Liang Li, Xiao-Yue Lv, Jia-Hong Duan, Xiao-Ping Liao & Ya-Hong Liu

  3. Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, 50011, Iowa, USA

    Qijing Zhang

  4. Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, 310027, Zhejiang, China

    Youjun Feng

  5. Jiangsu Co-Innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, Jiangsu, China

    Ya-Hong Liu

Authors
  1. Jian Sun
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Contributions

J.S., R.-S.Y., Q.Z., Y.F., X.-P.L. and Y.-H.L. designed the study and prepared the manuscript. R.-S.Y., L.-X.F., J.X., L.L., X.-Y.L. and J.-H.D. performed experiments. All authors discussed the results. J.S., R.-S.Y., Q.Z., Y.F., X.L. and Y.-H.L. analysed data. J.S., Q.Z., Y.F. and Y.-H.L. drafted this manuscript.

Corresponding authors

Correspondence to Xiao-Ping Liao or Ya-Hong Liu.

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Competing interests

The authors declare no competing financial interests.

Supplementary information

Supplementary information

Supplementary Figures 1-3, Supplementary Tables 1-2 (PDF 1331 kb)

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Sun, J., Yang, RS., Zhang, Q. et al. Co-transfer of blaNDM-5 and mcr-1 by an IncX3–X4 hybrid plasmid in Escherichia coli. Nat Microbiol 1, 16176 (2016). https://doi.org/10.1038/nmicrobiol.2016.176

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