Protein-synthesizing bacterial and archaeal cells can now be visualized by an adaptation of the BONCAT method, and sorted from complex samples for sequencing. A demonstration on the uncultivated, slow-growing methane-oxidizing consortia shows the high potential of this new method.
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Boetius, A. Microbial ecology: Seeing growth without culture. Nat Microbiol 1, 16158 (2016). https://doi.org/10.1038/nmicrobiol.2016.158
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DOI: https://doi.org/10.1038/nmicrobiol.2016.158