Species in the collection were used to query all 16S rRNA gene amplicon data sets stored in the SRA using IMNGS (www.imngs.org), as described in detail in the text. a, Percentage of samples from main ecological categories for which cumulative abundance of miBC species covered >1 or 10% total reads. b, As in a, including human and mouse gut samples only. Additional cumulative abundance categories are shown. c, Species-specific prevalence analysis in human and mouse gut samples. Black horizontal bars indicate median values. The 76 species in the collection were categorized as described in the Methods. The 18 bacteria selected for creation of the Minimal Bacteriome (MIBAC-1) are marked with asterisks. These species were selected based on their prevalence and abundance using both the MGS- and universal 16S rRNA gene amplicon-based surveys described in the text and also according to their phylogenetic diversity. Quotation marks indicate novel bacterial names proposed in the present study.