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Nanoscale resolution in GFP-based microscopy


We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The 70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results mark the advent of nanoscale biological microscopy with genetically encoded markers.

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Figure 1: STED microscope for GFP imaging.
Figure 2: STED overcomes the diffraction resolution barrier in GFP-based microscopy.
Figure 3: Subdiffraction imaging of GFP-labeled ER in PtK2 cell (ad) Confocal (a) and STED (b) images.


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Purified GFP-VLPs were provided by J. Cohen and the plasmid eGFP-ER by P. Lipp. We thank S. Verrier, T. Rosenmund, A.C. Schauss, J.J. Sieber and T. Müller for providing samples, A. Schönle for help with the software ImSpector, V. Westphal and B. Harke for valuable discussions, as well as J. Keller and B. Rankin for critical reading. We thank R.Y. Tsien for providing the plasmids coding for mCitrine, mStrawbery and mRFP.

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Correspondence to Stefan W Hell.

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Willig, K., Kellner, R., Medda, R. et al. Nanoscale resolution in GFP-based microscopy. Nat Methods 3, 721–723 (2006).

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