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Removal of a time barrier for high-resolution multidimensional NMR spectroscopy


We introduce the recursive multidimensional decomposition (R-MDD) method to speed recording of high-resolution NMR spectra. The measurement time is logarithmically dependent on the sizes of indirect spectral dimensions. R-MDD has the sensitivity and resolution advantages of optimized nonuniform acquisition schemes and is applicable to all types of biomolecular spectra. We demonstrated it for triple resonance experiments on three globular proteins (ubiquitin, azurin and the barstar-barnase complex) of 8–22 kDa.

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Figure 1
Figure 2: High resolution in the R-MDD reconstructions.


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This work was supported by grants from the Swedish Foundation for Strategic Research (A3 04:160d), the Swedish National Allocation Committee (SNIC 3/04-44), the Swedish Research Council (621-2005-2951) and the Wenner-Gren Foundation. The authors are grateful to G. Karlsson and S. Grzesiek for the azurin and ubiquitin samples.

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Authors and Affiliations



V.Yu.O. and V.J., idea of R-MDD algorithm and its demonstration for NMR; I.I., development of MDD algorithm for real and complex multidimensional sparse matricies.

Corresponding author

Correspondence to Vladislav Yu Orekhov.

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The authors declare no competing financial interests.

Supplementary information

Supplementary Fig. 1

Optimal sampling schedule for acquisition of high-resolution 3D HNCA spectrum of azurin. (PDF 154 kb)

Supplementary Fig. 2

Comparison with the reference of individual resonance reconstructions for 3D HNCA of azurin. (PDF 1337 kb)

Supplementary Fig. 3

Comparison with the reference of individual resonance reconstructions for 3D HNCO of barnase in complex with barstar. (PDF 475 kb)

Supplementary Fig. 4

Comparison with the reference of individual resonance reconstructions for 3D HNCO and 3D HNcoCA of ubiquitin. (PDF 673 kb)

Supplementary Fig. 5

Accuracy of intensities and peak positions between 6% data reconstruction and reference spectra for all backbone 3D HNCA correlations of protein azurin. (PDF 89 kb)

Supplementary Table 1

The samples and spectral parameters of the 3D experiments. (PDF 62 kb)

Supplementary Methods (PDF 181 kb)

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Jaravine, V., Ibraghimov, I. & Yu Orekhov, V. Removal of a time barrier for high-resolution multidimensional NMR spectroscopy. Nat Methods 3, 605–607 (2006).

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