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Enrichment of super-sized resequencing targets from the human genome

Nature Methods volume 4, pages 891–892 (2007)Cite this article

Methods relying on dense arrays of synthetic oligodeoxynucleotides to target specific subsets of the human genome may enable routine resequencing of all human exons or multi-megabase-pair chromosomal regions.

DNA sequencing should be thought of as a process rather than a method. Different steps in the process have been rate-limiting at different times during the past 30 years. Presently, at least for resequencing applications, the central bottleneck is acquisition of sequencing templates from targeted, multi-megabase-pair subsets of the billions of base pairs of DNA present in the human and other large genomes. Three papers in this issue of Nature Methods raise the hope that this bottleneck is about to disappear1,2,3.

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Figure 1: Two ways to use high-density arrays of custom-synthesized oligodeoxynucleotides to target subsets of the human genome.

Kim Caesar

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  1. Department of Genome Science and Medicine, Maynard Olson is at the University of Washington, Seattle, Washington 98195, USA. mvo@u.washington.edu,

    Maynard Olson

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  1. Maynard Olson
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Olson, M. Enrichment of super-sized resequencing targets from the human genome. Nat Methods 4, 891–892 (2007). https://doi.org/10.1038/nmeth1107-891

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