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Low- to high-throughput analysis of telomerase modulators with Telospot

Abstract

We designed a method termed Telospot to discover and characterize telomerase modulators as anticancer drugs or chemical biology tools. Telospot is based on a highly efficient human telomerase expression system and the detection of telomerase DNA reaction products in macroarray format. Telospot offers a highly scalable, cost- and time-effective alternative to presently available telomerase assays, which are limited by the requirement for PCR, telomerase purification or technologies not amenable to high throughput.

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Figure 1: Principle and implementation of Telospot.
Figure 2: Characterization of the hit compounds.

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Acknowledgements

We thank M. Chang for critical reading of the manuscript, H. Lashuel for generously providing the chemical library and K. Damm (Boehringer-Ingelheim) for providing BIBR1532. This work was supported by an European Molecular Biology Organization Long-Term Fellowship to G.C., grants from the Swiss Cancer League, the Swiss National Science Foundation, the Human Frontier Science Program and the EU 6th Framework Programme to J.L. and by the Ecole Polytechnique Fédérale de Lausanne to the Biomolecular Sceening Facility.

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Contributions

G.C. and P.R. performed the experiments; P.-O.R. implemented automation of the screen on the Biomek 3000; D.B. performed the statistical analysis; M.C. and G.T. designed the high-throughput screen; G.C. and J.L. designed the other experiments and wrote the manuscript.

Corresponding author

Correspondence to Joachim Lingner.

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The authors declare no competing financial interests.

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Supplementary Figure 1, Supplementary Tables 1–2, Supplementary Methods (PDF 198 kb)

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Cristofari, G., Reichenbach, P., Regamey, PO. et al. Low- to high-throughput analysis of telomerase modulators with Telospot. Nat Methods 4, 851–853 (2007). https://doi.org/10.1038/nmeth1099

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