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A model system for analyzing somatic mutations in Drosophila melanogaster

Abstract

Presently there are no good assays for comparing somatic mutation frequencies and spectra between different vertebrate and invertebrate organisms. Here we describe a new lacZ mutation reporter system in D. melanogaster, which complements existing systems in the mouse. The results obtained with the new model indicate two-to threefold higher frequencies of spontaneous mutations than in the mouse, with most of the mutations characterized as large genome rearrangements.

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Figure 1: Schematic depiction of the pUR288-S mutation reporter model in D. melanogaster.
Figure 2: Mutant frequencies and spectra in D. melanogaster.

References

  1. 1

    Vijg, J. Aging of the Genome (Oxford University Press, Oxford, 2007).

    Book  Google Scholar 

  2. 2

    Russell, L.B., Selby, P.B., von Halle, E., Sheridan, W. & Valcovic, L. Mutat. Res. 86, 355–379 (1981).

    CAS  Article  PubMed  Google Scholar 

  3. 3

    Kaya, B., Marcos, R., Yanikoglu, A. & Creus, A. Mutat. Res. 557, 53–62 (2004).

    CAS  Article  PubMed  Google Scholar 

  4. 4

    Bielas, J.H. & Loeb, L.A. Nat. Methods 2, 285–290 (2005).

    CAS  Article  PubMed  Google Scholar 

  5. 5

    Castro, J.P. & Carareto, C.M. Genetica 121, 107–118 (2004).

    CAS  Article  PubMed  Google Scholar 

  6. 6

    Ranz, J.M., Casals, F. & Ruiz, A. Genome Res. 11, 230–239 (2001).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  7. 7

    Curry, J., Karnaoukhova, L., Guenette, G.C. & Glickman, B.W. Genetics 152, 1065–1077 (1999).

    CAS  PubMed  PubMed Central  Google Scholar 

  8. 8

    Busuttil, R.A., Rubio, M., Dollé, M.E., Campisi, J. & Vijg, J. DNA Repair (Amst.) 5, 52–60 (2006).

    CAS  Article  Google Scholar 

  9. 9

    Dollé, M.E. et al. Mutat. Res. 596, 22–35 (2006).

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

We thank P. Kapahi for critical comments and valuable suggestions. This work was supported by a grant from the US National Institutes of Health (AG20438).

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Authors

Contributions

J.V. conceived, designed and supervised the study (initially with M.E.T.D.), obtained the funding and took the primary role in writing the manuscript. A.M.G. designed and performed most of the experiments, with A.D., assisted by R.B. and E.P. M.L. generated the transgenic flies (assisted by L.C.) and supervised the final experiments. R.B.C. provided statistical support.

Corresponding author

Correspondence to Jan Vijg.

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Competing interests

The authors declare no competing financial interests.

Supplementary information

Supplementary Fig. 1

Complete pCasper/pUR288-S nucleotide sequence. (PDF 47 kb)

Supplementary Fig. 2

Background mutant frequencies and spectra. (PDF 224 kb)

Supplementary Table 1

Sequenced size-change mutants recovered from line 11. (PDF 39 kb)

Supplementary Methods (PDF 73 kb)

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Garcia, A., Derventzi, A., Busuttil, R. et al. A model system for analyzing somatic mutations in Drosophila melanogaster. Nat Methods 4, 401–403 (2007). https://doi.org/10.1038/nmeth1027

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