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YFP photoconversion revisited: confirmation of the CFP-like species

Nature Methods volume 4, pages 767–768 (2007)Cite this article

To the editor:

The generation of a fluorescent byproduct of the photobleaching of yellow fluorescent protein (YFP) was first reported in Valentin et al.1. This finding brought into question conclusions of many fluorescence resonance energy transfer (FRET) studies that relied on an observed increase in cyan fluorescent protein (CFP) fluorescence upon photobleaching of the acceptor YFP. Others have not found such a byproduct2,3.

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Figure 1: COS cell expressing YFP PTP1B before and after bleaching with 514.

References

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Acknowledgements

We acknowledge support from the Whitaker Foundation, the Evans Foundation (M.T.K.), US National Institutes of Health grants AG027081 and HL081587 (J.F.K.) and a Scientist Development Grant from the American Heart Association (K.C.). L. Samelson (National Cancer Institute, National Institutes of Health) provided the gene encoding the CFP tagged EGF receptor. Z.-Y. Zhang (Indiana University School of Medicine) provided pJ3H-PTP1B (D181A/Q262A) encoding mutant PTP1B. The two-photon confocal microscope was designed and built in-house in collaboration with the lab of P. So (Massachusetts Institute of Technology).

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Authors and Affiliations

  1. Department of Medicine and Whitaker Cardiovascular Institute Multi-photon Microscopy Core, Section of Molecular Medicine, Boston University School of Medicine, Boston, 02118, Massachusetts, USA

    Michael T Kirber

  2. Division of Cardiovascular Medicine, Department of Medicine, University of Massachusetts Medical School, Worcester, 01655, Massachusetts, USA

    Kai Chen & John F Keaney Jr

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  1. Michael T Kirber
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  2. Kai Chen
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  3. John F Keaney Jr
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Kirber, M., Chen, K. & Keaney, J. YFP photoconversion revisited: confirmation of the CFP-like species. Nat Methods 4, 767–768 (2007). https://doi.org/10.1038/nmeth1007-767

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