Nat. Methods 11, 267–279 (2014); published online 27 February 2014; corrected after print 23 July 2014

In the version of this article initially published, 3D DAOSTORM was erroneously characterized in Table 2 as using least-squares fits. In fact, 3D DAOSTORM uses an implementation of maximum likelihood estimation (MLE). The error has been corrected in the HTML and PDF versions of the article.