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Hold me tightly LOV

Mutations that increase the dynamic range of a photoswitchable protein may be used to improve other such photoswitches and increase their potential for allosteric control of protein activity in live cells.

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Figure 1: LOV domain–based photoswitches.

References

  1. 1

    Strickland, D. et al. Nat. Methods 7, 623–626 (2010).

    CAS  Article  Google Scholar 

  2. 2

    Liu, C.C. & Schultz, P.G. Annu. Rev. Biochem. 79, 413–444 (2010).

    CAS  Article  Google Scholar 

  3. 3

    Airan, R.D., Thompson, K.R., Fenno, L.E., Bernstein, H. & Deisseroth, K. Nature 458 (7241), 1025–1029 (2009).

    Article  Google Scholar 

  4. 4

    Levskaya, A., Weiner, O.D., Lim, W.A. & Voigt, C.A. Nature 461, 997–1001 (2009).

    CAS  Article  Google Scholar 

  5. 5

    Wu, Y.I. et al. Nature 461, 104–108 (2009).

    CAS  Article  Google Scholar 

  6. 6

    Yoo, S.K. et al. Dev. Cell 18, 226–236 (2010).

    CAS  Article  Google Scholar 

  7. 7

    Lee, J. et al. Science 322, 438–442 (2008).

    CAS  Article  Google Scholar 

  8. 8

    Strickland, D., Moffat, K. & Sosnick, T.R. Proc. Natl. Acad. Sci. USA 105, 10709–10714 (2008).

    CAS  Article  Google Scholar 

  9. 9

    Yao, X., Rosen, M.K. & Gardner, K.H., Nat. Chem. Biol. 4, 491–497 (2008).

    CAS  Article  Google Scholar 

  10. 10

    Christie, J.M. et al. Biochemistry 46, 9310–9319 (2007).

    CAS  Article  Google Scholar 

  11. 11

    Zoltowski, B.D. Vaccaro, B. & Crane, B.R. Nat. Chem. Biol. 5, 827–834 (2009).

    CAS  Article  Google Scholar 

Download references

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Correspondence to Klaus M Hahn or Brian Kuhlman.

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Hahn, K., Kuhlman, B. Hold me tightly LOV. Nat Methods 7, 595–596 (2010). https://doi.org/10.1038/nmeth0810-595

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